Results 181 to 190 of about 53,634 (224)

PCR Methods for the Generation of Catalytic ssDNA

2022
The ability to produce single-stranded DNA on a preparative scale from low amounts of starting templates is necessary for most research involving deoxyribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of
Deni, Szokoli, Kristian K, Le Vay, Hannes, Mutschler   +2 more
openaire   +2 more sources

ssDNA Templated Self-Assembly of Chromophores

Journal of the American Chemical Society, 2007
A single strand of DNA templates a supramolecular strand of chromophores, yielding a new type of DNA hybrid. In this hybrid construct, an oligothymine is used as the template to which a supramolecular stack of naphthalenes or oligo(phenylene)vinylenes equipped with a complementary diamino triazine unit is hydrogen ...
Janssen, P.G.A., Vandenbergh, J., Dongen, van, J.L.J., Meijer, E.W., Schenning, A.P.H.J.   +4 more
openaire   +3 more sources

Modeling of the [E43S]SNase-ssDNA–Cd2+ complex: Structural insight into the action of nuclease on ssDNA

Archives of Biochemistry and Biophysics, 2013
Staphylococcal nuclease (SNase) catalyzes the hydrolysis of the phosphate backbone of DNA and RNA leaving 3'-phosphate mononucleotides and dinucleotides. SNase has been extensively used as a model protein for investigating enzymatic mechanism, thermodynamic stability, and protein folding.
Tao, Xie, Yingang, Feng, Lu, Shan, Jinfeng, Wang   +3 more
openaire   +2 more sources

Anti‐ssDNA and dsDNA Antibodies in Preeclampsia

Asia-Oceania Journal of Obstetrics and Gynaecology, 1994
AbstractThe presence of anti‐ssDNA and anti‐dsDNA antibodies and their clinical significance in preeclampsia were studied. Serum samples were taken from 38 cases of preeclampsia including 19 severe cases, and from 26 normal pregnant women. Anti‐ssDNA and anti‐dsDNA antibodies were measured by using an enzyme‐linked im‐munosorbent assay (ELISA).Only 1 ...
T, Yamamoto, S, Yoshimura, Y, Geshi, Y, Sasamori, H, Mori, T, Kobayashi   +5 more
openaire   +2 more sources

Biclustering for ssDNA aptamer motif prototypes

2016 IEEE-EMBS International Conference on Biomedical and Health Informatics (BHI), 2016
Aptamers and biclustering are enabling technologies for personalized medicine. Aptamers are highly valuable for medicine and biosensors, but the search is lengthy. We seek to accelerate aptamer discovery by reporting on the first set of DNA aptamer biclusters, which are based on a database of known DNA aptamers.
Erin J. Moore, Thirimachos Bourlai
openaire   +1 more source

ssDNA aptamers that selectively bind oxytetracycline

Bioorganic & Medicinal Chemistry, 2008
Single stranded DNA aptamers that bind with high affinity and specificity to the oxytetracycline (OTC) were identified by selection from an oligonucleotide library of 10(15) molecules. The binding affinities of four aptamers were in nanomolar range. The aptamers were highly selective in that, lack of -OH group at 5-position in tetracycline and -H group
Javed H, Niazi, Su Jin, Lee, Yeon Seok, Kim, Man Bock, Gu   +3 more
openaire   +2 more sources

Molecular dynamics simulation of ssDNA and cationic polythiophene

Journal of Molecular Graphics and Modelling, 2023
In this work, molecular dynamics simulations of complexes composed of single strand DNA (ssDNA) sequences and cationic oligothiophenes are performed to understand experimental findings and the sensing ability of polythiophene electrolytes toward ssDNA.
Nehir Nalıncı Barbak, Erman Kıbrıs, Fethi Can Demirci, Nuran Elmacı Irmak   +3 more
openaire   +2 more sources

Selection and Application of ssDNA Aptamers against Clenbuterol Hydrochloride Based on ssDNA Library Immobilized SELEX

Journal of Agricultural and Food Chemistry, 2017
Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA ...
Nuo Duan, Wenhui Gong, Shijia Wu, Zhouping Wang   +3 more
openaire   +2 more sources