Results 191 to 200 of about 7,066 (236)
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Nature Protocols, 2007
Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. Stable-isotope-labeled carbon (13C) or nitrogen (15N) sources are assimilated into microbial biomass of environmental samples ...
Neufeld, Josh D. +6 more
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Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. Stable-isotope-labeled carbon (13C) or nitrogen (15N) sources are assimilated into microbial biomass of environmental samples ...
Neufeld, Josh D. +6 more
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Protein-based stable isotope probing
Nature Protocols, 2010We describe a stable isotope probing (SIP) technique that was developed to link microbe-specific metabolic function to phylogenetic information. Carbon ((13)C)- or nitrogen ((15)N)-labeled substrates (typically with >98% heavy label) were used in cultivation experiments and the heavy isotope incorporation into proteins (protein-SIP) on growth was ...
Nico, Jehmlich +7 more
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Stable isotope probing — linking microbial identity to function
Nature Reviews Microbiology, 2005Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis ...
Marc Dumont, Murrell J Colin
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Stable Isotope Probing and Plants
2014This chapter summarizes the applications of stable isotope probing (SIP) technology in plant-soil systems and presents an overview of the progress achieved in the understanding of the plant-soil microbe interactions and their role in ecosystem functioning.
Lu, Y., Conrad, R.
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Stable-isotope probing as a tool in microbial ecology
Nature, 2000Microorganisms are responsible for driving the biogeochemical cycling of elements on Earth. Despite their importance and vast diversity, the taxonomic identity of the microorganisms involved in any specific process has usually been confined to that small fraction of the microbiota that has been isolated and cultivated.
S, Radajewski +3 more
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DNA-Based Stable Isotope Probing
2019Microbiomes on Earth are often considered the most heterogeneous biological entities, but their vital roles in driving global biogeochemical cycles often remain elusive. DNA-based stable isotope probing (DNA-SIP) provides a powerful means to establish a direct link between biogeochemical processes and the taxonomic identities of active microorganisms ...
Zhongjun, Jia +2 more
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Stable isotope probing in biodegradation research
Trends in Biotechnology, 2004Stable isotope probing (SIP), coupled with advances in genomics and analytical chemistry, is leading to major progress in biodegradation research. A recent paper describes the use of SIP to determine the primary member of a complex community that is responsible for naphthalene catabolism in situ.
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Stable Isotope Probing and Metagenomics
2014Two promising culture-independent approaches that have been employed to assess the function and metabolic potential of uncultivated microorganisms are stable isotope probing (SIP) and metagenomics. This chapter discusses the methodology of metagenomics within the context of DNA stable isotope probing (DNA-SIP), and provides a description of the ...
Lee J. Pinnell +2 more
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RNA Stable Isotope Probing (RNA-SIP)
2019Stable isotope probing is a combined molecular and isotopic technique used to probe the identity and function of uncultivated microorganisms within environmental samples. Employing stable isotopes of common elements such as carbon and nitrogen, RNA-SIP exploits an increase in the buoyant density of RNA caused by the active metabolism and incorporation ...
Noor-Ul-Huda, Ghori +6 more
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Protein Stable Isotope Probing
2014The chapter on protein stable isotope probing (protein-SIP) focuses on cultivation conditions, extraction of proteins, different protein-separation techniques, methods of mass spectrometry (MS) analysis, and the calculation of stable isotope incorporation levels.
Nico Jehmlich +6 more
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