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Fluorescent Staining of Bacteria: Viability and Antibody Labeling
Current Protocols in Microbiology, 2009AbstractThis appendix presents several methods for using fluorescence to evaluate bacterial viability and to explore the cell surface for the presence of various antigens for diagnostic and taxonomic purposes. The use of fluorescent labeling will allow fast and accurate analysis and monitoring of microbial populations in ecological and clinical ...
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Microorganisms in soil as observed by staining with rhodamine-labeled lysozyme
Canadian Journal of Microbiology, 1970Indigenous soil microorganisms on soil contact slides were stained with rhodamine-labeled lysozyme and counterstained with fluorescein-labeled gelatin. Bacteria and actinomycetes on these slide preparations were readily observed by fluorescence microscopy even if they occurred on or near soil particles.
W N, Millar, L E, Casida
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Fluorochrome-Labeled Deoxyribonuclease: Specific Stain for Cell Nuclei
Science, 1968Nuclei of cells in tissue culture may be stained with deoxyribonuclease labeled with fluorescein isothiocyanate; this is a simple, rapid, and specific process which is useful in localizing DNA in situ .
M A, Benjaminson, D B, Hunter, I J, Katz
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Digital Staining of Mitochondria in Label-free Live-cell Microscopy
2021Examining specific sub-cellular structures while minimizing cell perturbation is important in the life sciences. Fluorescence labeling and imaging is widely used. With the advancement of deep learning, digital staining routines for label-free analysis have emerged to replace fluorescence imaging. Nonetheless, digital staining of sub-cellular structures
Ayush Somani +8 more
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Virtual Staining of Label-free Tissue
Frontiers in Optics + Laser Science 2024 (FiO, LS)We will cover deep learning-based virtual staining techniques that can generate different types of histological stains from label-free microscopic images of unstained samples by using, e.g., autofluorescence microscopy, quantitative phase imaging and reflectance confocal microscopy.
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Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels
Journal of Immunological Methods, 1985Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers.
J J, Van Dongen +5 more
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Ferritin Labeled Antibody Staining of Thin Sections
Proceedings, annual meeting, Electron Microscopy Society of America, 1969The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb
J. D. McLean, S. J. Singer
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Intensification of labelings of the immunogold silver staining method by gold toning
Brain Research Bulletin, 1992We evaluated the applicability of the gold toning procedure to the immunogold silver staining method using monoclonal antibody against dopamine. Immunolabeling was examined in the rat substantia nigra at light and electron microscopic levels. Vibratome sections of fixed midbrains were incubated with anti-dopamine antiserum and then with 5 nm colloidal ...
R, Arai, M, Geffard, A, Calas
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Virtual staining of label-free tissue using deep learning
Advanced Chemical Microscopy for Life Science and Translational Medicine 2026, 2023We will report deep learning-based virtual staining techniques to generate various histological stains, including immunohistochemical (IHC) stains, from label-free microscopic images of tissue samples by using autofluorescence microscopy, quantitative phase imaging (QPI) and reflectance confocal microscopy.
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Patterned Staining by Fluorescein-Labeled Oligonucleotides Obtained by in Vitro Selection
Analytical Chemistry, 1996We describe a new means of surface staining using a fluorescent oligonucleotide. A folic acid-specific, fluorescent-labeled oligonucleotide was selected from a group of randomly sequenced oligonucleotides by repeated elution through a folic acid-immobilized column and amplified by the polymerase chain reaction.
N, Kawazoe, Y, Ito, Y, Imanishi
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