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Enzyme-Labeling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining

Journal of Immunoassay, 1983
Abstract The use of an enzyme as a label has a number of advantages over the use of other labels in both immunohistochemistry and immunoassay. Immunofluorescence techniques are not suitable for ultrastructural research on cells, and ferritin-labeled antibodies allow only electronmicroscopic studies.
Eiji Ishikawa   +2 more
exaly   +3 more sources

Efficient Cell Labelling for Gram Stain WSIs

2022 26th International Conference on Pattern Recognition (ICPR), 2022
The Gram stain test is one of the most commonly used procedures in microbiology labs as the first step to diagnose infection. Automated interpretation of such a test can be very beneficial, but the difficulty of obtaining fully annotated Gram stain Whole Slide Images (WSIs) datasets has hindered research in the area.
Sarah Alhammad   +5 more
openaire   +3 more sources

Chameleon Labels for Staining and Quantifying Proteins

Angewandte Chemie International Edition, 2004
Glowing marks: A new class of protein stains, the pyrylium dyes, undergo a strong color change (typically from blue to red, see picture) on covalently binding to proteins. While the free stains are almost nonfluorescent, the protein-conjugated forms are highly fluorescent.
Bianca K, Wetzl   +3 more
openaire   +2 more sources

Quantitative Gram-staining with Labelled Iodine

Nature, 1958
THE development of a simple quantitative Gram staining technique has been attempted by numerous observers1,2, not only for its possible practical uses but also because of its potential value in studies on the mechanism of the staining reaction on intact cells and on extracellular material3, as well as the relationship between degree of Gram-positivity ...
D, SHUGAR, J, BARANOWSKA
openaire   +2 more sources

Post-embedding Staining with Ferritin Labelled Antibodies

Nature, 1967
THE ferritin labelled antibody technique1 has been widely used for the location of antigens at the cellular and sub-cellular level2. There are two possible methods of applying the technique: (a) pre-embedding staining in which antigen is allowed to react with the labelled antibody before the cells are processed and sectioned in preparation for electron
R O, Thomson   +3 more
openaire   +2 more sources

Elective vital staining of mouse tumors with labelled dyes

Medical Hypotheses, 1981
It was shown by vital staining in previous experiments (1) that carcinoma and sarcoma of mice could be selectively stained by certain dyes of the triphenyl methane sulphonic acid group. These findings were now confirmed in the present experiments by using labelled (125I) dyes of the same chemical group.
D, Engel, I, Benes
openaire   +2 more sources

Immunohistochemical staining of bromodeoxyuridine‐labelled cells in the mouse embryo

Journal of Microscopy, 1989
SUMMARYThe aim of this study was to compare two immunohistochemical methods, avidin‐biotin peroxidase and immunogold silver staining (IGSS), in the detection of 5‐bromo‐deoxyuridine incorporation in mouse embryo tissues. In addition, two fixation schedules, formal‐saline and a mixture of formaldehyde and glutaraldehyde (4F1G), and two embedding ...
D C, Cottell   +2 more
openaire   +2 more sources

A Method for Automatic Counting and Labeling of Cells Stained with Microporous Membrane

2021 IEEE 16th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), 2021
Cells in the blood can be screened through a membrane. But the method of manually identifying the number of cells is time-consuming and laborious, and there may even be some errors. Therefore, an effective image recognition method was adopted in this paper to automatic count and label the stained cells on the membrane after filtration.
Jiangcheng Cao   +5 more
openaire   +1 more source

Staining of human telomeres with primed in situ labeling (PRINS)

Cytogenetics and Cell Genetics, 2008
As described, the PRINS method is a very rapid and reliable way of staining human telomeres. To obtain the maximum frequency of stained telomeres, the primer (CCCTAA)7 should be used, although the average frequency never quite reaches 100 %. The frequency is strongly dependent on the age of the individual, being significantly higher in children and ...
A J, Therkelsen   +4 more
openaire   +2 more sources

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