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Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes
Proceedings, annual meeting, Electron Microscopy Society of America, 1995We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br
R. Levi-Setti +3 more
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Comparison of Findings of the Bone Stain and Tetracycline Labeling in Monkey Ribs
Journal of Histotechnology, 1978Abstract An attempt was made to interpret the chromatic properties of osteoid seams and tetracycline labels in Rhesus monkey bone. Observation included counting the total number of different types of osteoid seams shown by the Bone stain, and the total number of tetracycline labels.
C. C. Schock +2 more
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Journal of Immunoassay and Immunochemistry, 2010
A comparative analysis was performed to determine the sensitivity and efficiency of three fluorescent labeling techniques, including direct fluorescent-antibody staining (FA), Zenon labeling, and quantum dot (QD) nanocrystal technology. Two varicella-zoster virus immunoglobin (Ig) G forms, mAb 4F9 and mAb g62, were selected for these studies.
Xiaoling, Tang +3 more
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A comparative analysis was performed to determine the sensitivity and efficiency of three fluorescent labeling techniques, including direct fluorescent-antibody staining (FA), Zenon labeling, and quantum dot (QD) nanocrystal technology. Two varicella-zoster virus immunoglobin (Ig) G forms, mAb 4F9 and mAb g62, were selected for these studies.
Xiaoling, Tang +3 more
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Journal of Immunological Methods, 1975
A technique for the immunoferritin labelling of antibody participating in virus--antibody complexes has been developed. Complexes were labelled by incubation with the immunoferritin reagent and excess reagent removed by polyacrylamide gel electrophoresis. Labelled complexes were then visualised in the electron microscope by negative staining.
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A technique for the immunoferritin labelling of antibody participating in virus--antibody complexes has been developed. Complexes were labelled by incubation with the immunoferritin reagent and excess reagent removed by polyacrylamide gel electrophoresis. Labelled complexes were then visualised in the electron microscope by negative staining.
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Combined Ki-67 and feulgen stain for morphometric determination of the Ki-67 labelling index
Histochemistry, 1993In this note we present a combined Ki-67 and Feulgen stain for morphometric determination of the Ki-67 labelling index. The immunohistochemical part of this double staining technique is based on the alkaline-phosphatase-anti-alkaline-phosphatase (APAAP) method, visualizing the enzyme activity by the nitro-blue-tetrazolium chloride (NBT)/bromo-chloro-3 ...
H, Kolles +3 more
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Direct and Indirect Staining of Bacteria with Fluorescein-Labeled Antibodies
1979The two most frequently used fluorescent procedures for the localization of antigens in different preparations are the direct and indirect methods. In direct staining, the antibody which has been produced in experimental animals or isolated from human serum is directly labeled with fluorescein. In the indirect method, the antibody which will react with
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The Journal of Comparative Neurology, 1999
The AII or rod amacrine cell is a critical interneuron in the rod pathway of mammalian retinae. In this report, it is shown that commercially available antibodies to the calcium binding protein calretinin may be used to label the population of AII amacrine cells selectively.
S C, Massey, S L, Mills
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The AII or rod amacrine cell is a critical interneuron in the rod pathway of mammalian retinae. In this report, it is shown that commercially available antibodies to the calcium binding protein calretinin may be used to label the population of AII amacrine cells selectively.
S C, Massey, S L, Mills
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Aquatic Botany, 2007
Abstract In most red algae, spores are liberated without a cell wall, within a sheath of mucilage that is responsible for its primary attachment. Utilizing fluorescent-labelled lectins, we identified carbohydrate residues and their location in the mucilage and cell walls of spores of Laurencia arbuscula . Cell wall formation and mucilage composition
Zenilda Laurita Bouzon +1 more
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Abstract In most red algae, spores are liberated without a cell wall, within a sheath of mucilage that is responsible for its primary attachment. Utilizing fluorescent-labelled lectins, we identified carbohydrate residues and their location in the mucilage and cell walls of spores of Laurencia arbuscula . Cell wall formation and mucilage composition
Zenilda Laurita Bouzon +1 more
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The American journal of pathology, 1991
Monoclonal antibodies (MAbs) to nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative characteristics of various types of cancer. Prerequisite for the application of these MAbs in surgical pathology is establishment of the level of expression and/or cellular distribution of the antigens in relation ...
J H, van Dierendonck +4 more
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Monoclonal antibodies (MAbs) to nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative characteristics of various types of cancer. Prerequisite for the application of these MAbs in surgical pathology is establishment of the level of expression and/or cellular distribution of the antigens in relation ...
J H, van Dierendonck +4 more
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High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 2017
Visible absorption in tissue is dominated by a very small number of chromophores (hemoglobins and melanins) with broad optical spectra; for melanins in particular, the optical absorption spectrum is typically featureless. In addition, scattering limits penetration depth.
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Visible absorption in tissue is dominated by a very small number of chromophores (hemoglobins and melanins) with broad optical spectra; for melanins in particular, the optical absorption spectrum is typically featureless. In addition, scattering limits penetration depth.
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