Results 131 to 140 of about 463,138 (331)

Investigating the molecular mechanisms underlying the anti‐CRISPR function of AcrIIA13b protein

The FEBS Journal, EarlyView.
Anti‐CRISPR protein AcrIIA13b inhibits CRISPR–Cas9 function by preventing target DNA engagement. The Cas9–sgRNA ribonucleoprotein (RNP) normally recognizes the protospacer adjacent motif (PAM) duplex to initiate DNA cleavage. AcrIIA13b binding to the PAM‐interacting WED–PI domain blocks this recognition, leaving the target DNA intact.
So Yeon Lee, Hyun Ho Park
wiley   +1 more source

CRISPR-Cas9 enables efficient genome engineering of the strictly lytic, broad-host-range staphylococcal bacteriophage K

Applied and Environmental Microbiology
Staphylococcus aureus is a major opportunistic pathogen, increasingly difficult to treat due to rising resistance to methicillin, vancomycin, and other antimicrobials. Bacteriophages offer a promising alternative, particularly when conventional therapies
Jonas Fernbach, Jasmin Baggenstos, Ellen-Aleksandra Svorjova, Jeannine Riedo, Shawna McCallin, Martin J. Loessner, Samuel Kilcher   +6 more
doaj   +1 more source

Molecular Characterisation of Bacteriophage K Towards Applications for the Biocontrol of Pathogenic Staphylococci [PDF]

, 2006
End of project reportThe aim of this work was to characterise staphylococcal bacteriophage (a bacterial virus) and to assess their potential as therapeutic agents against pathogenic strains of Staphylococcus aureus, particularly mastitis-causing strains.
Coffey, Aidan, Fitzgerald, Gerald F, Flynn, James, Meaney, William J, O'Flaherty, Sarah, Ross, R Paul   +5 more
core  

Susceptibility of staphylococcus epidermidis planktonic cells and biofilms to the lytic action of staphylococcus bacteriophage K [PDF]

, 2007
Aims: To evaluate differences in biofilm or planktonic bacteria susceptibility to be killed by the polyvalent antistaphylococcus bacteriophage K. Methods and Results: In this study, the ability of phage K to infect and kill several clinical isolates of ...
Azeredo, Joana, Cerca, Nuno, Oliveira, Rosário   +2 more
core   +1 more source

Facing Antibiotic Resistance: Staphylococcus aureus Phages as a Medical Tool

Viruses, 2014
Staphylococcus aureus is a common and often virulent pathogen in humans. This bacterium is widespread, being present on the skin and in the nose of healthy people.
Z. Kaźmierczak, A. Górski, K. Dąbrowska   +2 more
semanticscholar   +1 more source

Properties of Staphylococcus Phage UC-18: A Comparison with the International Phage Series.

Experimental Biology and Medicine, 1966
SummaryPhage UC-18 was shown to have a latent period of 35 minutes and a burst size of 7 particles per cell. The phage had an average buoyant density of 1.508 g/cm3 and on electron microscopic examination the head was found to have a regular hexagonal silhouette (60 mμ in diameter) with a tail (165 mμ in length) terminating in a knob-like baseplate ...
L, Blouse, R, Twarog
openaire   +2 more sources

Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids

Molecular Oral Microbiology, EarlyView.
We report optimization of the Treponema denticola shuttle plasmid system by minimizing its size and by characterizing an array of promoter‐gene constructs for genetic complementation, including the first use in Treponema of a Tet‐inducible system for controlled expression of potentially toxic genes.
M. Paula Goetting‐Minesky, Valentina Godovikova, Prakaimuk Saraithong, Alexander H. Rickard, Brigette R. Crawley, Sara M. Agolli, Reagan L. Boyce, Trishna L. Appaji, J. Christopher Fenno   +8 more
wiley   +1 more source

Germicidal potential and skin compatibility of an innovative UVC phototherapy device emitting at 234 nm

Photochemistry and Photobiology, EarlyView.
A phototherapy device emitting principally at 234 nm was assessed for antimicrobial efficacy against Methicillin‐resistant Staphylococcus aureus and evaluated for skin health hazards. Abstract Chronic wounds are a major healthcare issue affecting more than 10 million Americans each year, with a 5‐year survival similar to cancer and costing the ...
Zheng Tang, David Welch, Manuela Buonanno, Mark Gerber, David J. Brenner   +4 more
wiley   +1 more source

Evaluation of Microencapsulation of The UFV-AREG1 Bacteriophage in Alginate-Ca Microcapsules using Microfluidic Devices

, 2017
The indiscriminate use of antibiotics and the emergence of resistant microorganisms have become a major challenge for the food industry. The purpose of this work was to microencapsulate the bacteriophage UFV-AREG1 in a calcium alginate matrix using ...
Batalha, Laís S., Boggionea, Delaine M. G., Gontijo, Marco T. P., Lopez, Maryoris E. S., Mendonça, Regina C. S., Santos, Igor J. B., Teixeira, Alvaro V. N. C.   +6 more
core   +2 more sources

Staphylococcus aureus controls interleukin-5 release in upper airway inflammation [PDF]

, 2018
Staphylococcus aureus is a frequent colonizer of the upper airways in chronic rhinosinusitis with nasal polyps, but also resides intramucosally, it has been shown that secreted staphylococcal proteins such as enterotoxins and serine proteases induce the ...
Bachert, Claus, Bröker, BM, Gevaert, Elien, Holtappels, Gabriële, Merabishvili, Maia, Meyer, T, Murr, A, Schmidt, F, Van Crombruggen, Koen, Vaneechoutte, Mario, Völker, U, Zhang, Nan   +11 more
core   +2 more sources