Results 161 to 170 of about 7,517,121 (308)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Accessing the density-free regime with ECRH-assisted ohmic start-up on EAST. [PDF]

open access: yesSci Adv
Liu J   +18 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

From Walking Simulator to Reflective Simulator: A Practice-Based Perspective

open access: yes, 2019
The aim of this paper is to contribute both theoretically and methodologically to discussions on walking simulator video games. We base our reflection on results grounded in a video game design project conducted as part of our master\u27s thesis.
Ferland-Beauchemin, Alexandra   +2 more
core  

Accelerating start-up cycles in investigator-initiated multicenter clinical trials. [PDF]

open access: yesJ Clin Transl Sci
Hillery S   +13 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Reading, Writing, Lexigraphing: Active Passivity as Queer Play in Walking Simulators

open access: yes, 2019
In this article, we address the histories and influences of reading and writing within the genre of digital games called “walking simulators.” Reading is framed as an activity separate from (and, sometimes, incompatible with) the set of actions afforded ...
Bohunicky, Kira, Milligan, Chloe Anna
core  

Home - About - Disclaimer - Privacy