Results 111 to 120 of about 2,035,474 (280)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

open access: yesFEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Numerical study of thermally stratified flows of a fluid overlying a highly porous material

open access: yes, 2014
In this talk we are concerned with thermally stratified flows in domains that contain a macroscopic interface between a highly porous material and a pure-fluid domain.
Antoniadis, Panagiotis   +2 more
core  

Calculation and Analysis of Wellbore Temperature Field in HTHP Gas Wells

open access: yesShiyou jixie, 2019
To ensure safe drilling of HTHP gas wells, it is necessary to study the wellbore temperature field in HTHP gas wells. Considering the heat transfer of wellbore fluid and formation, based on the thermodynamics law and the energy conservation law, the ...
Wang Ke, Liu Biao, Zhang Jun, Su Yu
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

21st Australasian Fluid Mechanics Conference (AFMC 2018)

open access: yes, 2018
Fluid Structure Interactions (FSI), if not managed appropriately are known to have contributed to the loss of several aerospace vehicles. As done for the X-43, FSI can be designed-out by making structures sufficiently rigid and by providing appropriate ...
Jacobs, P.   +4 more
core   +1 more source

Identification of Students' Mastery Level of Physics Concepts in Static Fluid Material

open access: yesJPFT (Jurnal Pendidikan Fisika dan Teknologi)
This study aims to identify the level of mastery of physics concepts on static fluid material. This research uses quantitative descriptive methods. The research subjects consisted of 23 MA students in Malang.
Fatmawati Liana Wulandari   +2 more
doaj   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

open access: yesFEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia   +9 more
wiley   +1 more source

Fluid-motion effects on the dynamics of thermal frontal polymerization

open access: yes
Thermal frontal polymerization (FP) is a process that rapidly converts liquid monomer solutions into solid polymers via self-sustaining exothermic reaction fronts. It is a promising method for polymer manufacturing because it is fast and energy-efficient.
78th APS Division of Fluid Dynamics Annual Meeting 2025   +2 more
core  

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