Results 111 to 120 of about 2,035,474 (280)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Numerical study of thermally stratified flows of a fluid overlying a highly porous material
In this talk we are concerned with thermally stratified flows in domains that contain a macroscopic interface between a highly porous material and a pure-fluid domain.
Antoniadis, Panagiotis +2 more
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Calculation and Analysis of Wellbore Temperature Field in HTHP Gas Wells
To ensure safe drilling of HTHP gas wells, it is necessary to study the wellbore temperature field in HTHP gas wells. Considering the heat transfer of wellbore fluid and formation, based on the thermodynamics law and the energy conservation law, the ...
Wang Ke, Liu Biao, Zhang Jun, Su Yu
doaj
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
21st Australasian Fluid Mechanics Conference (AFMC 2018)
Fluid Structure Interactions (FSI), if not managed appropriately are known to have contributed to the loss of several aerospace vehicles. As done for the X-43, FSI can be designed-out by making structures sufficiently rigid and by providing appropriate ...
Jacobs, P. +4 more
core +1 more source
Identification of Students' Mastery Level of Physics Concepts in Static Fluid Material
This study aims to identify the level of mastery of physics concepts on static fluid material. This research uses quantitative descriptive methods. The research subjects consisted of 23 MA students in Malang.
Fatmawati Liana Wulandari +2 more
doaj +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Fluid-motion effects on the dynamics of thermal frontal polymerization
Thermal frontal polymerization (FP) is a process that rapidly converts liquid monomer solutions into solid polymers via self-sustaining exothermic reaction fronts. It is a promising method for polymer manufacturing because it is fast and energy-efficient.
78th APS Division of Fluid Dynamics Annual Meeting 2025 +2 more
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