Results 201 to 210 of about 201,880 (241)
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Large Stokes-shift bioorthogonal probes for STED, 2P-STED and multi-color STED nanoscopy
Methods and Applications in Fluorescence, 2020Abstract Synthesis and multiple STED imaging applications of four, red-emitting (610–670 nm), tetrazine-functionalized fluorescent probes (CBRD = Chemical Biology Research group Dye 1–4) with large Stokes-shift is presented. Present studies revealed the super-resolution microscopy applicability of the probes as demonstrated through ...
György Török +8 more
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Proceedings of the 19th ACM SIGKDD international conference on Knowledge discovery and data mining, 2013
Social microblogs such as Twitter and Weibo are experiencing an explosive growth with billions of global users sharing their daily observations and thoughts. Beyond public interests (e.g., sports, music), microblogs can provide highly detailed information for those interested in public health, homeland security, and financial analysis.
Ting Hua +4 more
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Social microblogs such as Twitter and Weibo are experiencing an explosive growth with billions of global users sharing their daily observations and thoughts. Beyond public interests (e.g., sports, music), microblogs can provide highly detailed information for those interested in public health, homeland security, and financial analysis.
Ting Hua +4 more
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Intet steds udenfor. Intet sted.
Peripeti, 2016Essay af Florian Malzacher, hvor han beskriver tilstanden af teaterkritik i Tyskland efter århundredeskiftet.
Florian Malzacher +2 more
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Expansion STED microscopy (ExSTED)
2021The recently developed expansion microscopy method (ExM) allows for the resolution of structures below the diffraction limit of light not by sophisticated instrumentation, but rather by physically expanding the molecular structure of cells. This happens by crosslinking the protein in the sample to a hydrogel that is polymerized in situ and subsequently
Gao, M. +4 more
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Optik & Photonik, 2010
AbstractDie ultra‐hochauflösende Lichtmikroskopie hat den Lebenswissenschaften zu einem beachtlichen Sprung in ihrer Entwicklung verholfen. Noch vor wenigen Jahren war es für Biologen und Mediziner undenkbar, Strukturen kleiner als 100 nm aufzulösen oder gar dynamische Prozesse in lebenden Zellen zu beobachten. Die STED‐Mikroskopie (STimulated Emission
Jochen J. Sieber, Anja Schué
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AbstractDie ultra‐hochauflösende Lichtmikroskopie hat den Lebenswissenschaften zu einem beachtlichen Sprung in ihrer Entwicklung verholfen. Noch vor wenigen Jahren war es für Biologen und Mediziner undenkbar, Strukturen kleiner als 100 nm aufzulösen oder gar dynamische Prozesse in lebenden Zellen zu beobachten. Die STED‐Mikroskopie (STimulated Emission
Jochen J. Sieber, Anja Schué
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Nature Methods, 2016
The use of photoswitchable fluorescent proteins in STED nanoscopy enables higher-contrast imaging with dramatically reduced photobleaching.
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The use of photoswitchable fluorescent proteins in STED nanoscopy enables higher-contrast imaging with dramatically reduced photobleaching.
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Sub-Abbe resolution: from STED microscopy to STED lithography
Physica Scripta, 2014We review the emergence of three-dimensional sub-Abbe optical nanoscopy, which in its original version deployed stimulated-emission-induced depletion (STED) of the excited state of the fluorophores to break the diffraction limit. We focus first on different methods to realize a donut-shaped point spread function (PSF), which are key to distribute the ...
Thomas A Klar +2 more
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2014
The turn of the twenty-first century has witnessed the advent of far-field (lens-based) fluorescence nanoscopy, a fluorescence microscopy featuring a spatial resolution down to molecular scales. Lens-based optical microscopy is a very popular technique for investigating the living cell, but the spatial resolution of its standard versions is limited to ...
Christian Eggeling, Stefan W. Hell
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The turn of the twenty-first century has witnessed the advent of far-field (lens-based) fluorescence nanoscopy, a fluorescence microscopy featuring a spatial resolution down to molecular scales. Lens-based optical microscopy is a very popular technique for investigating the living cell, but the spatial resolution of its standard versions is limited to ...
Christian Eggeling, Stefan W. Hell
openaire +1 more source