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Brassinosteroids in Cucurbits: Modulators of Plant Growth Architecture and Stress Response. [PDF]
Słomnicka R +3 more
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Studies on adrenal steroid hydroxylases. Complex formation of the hydroxylase components.
J W, Chu, T, Kimura
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Steroid hormones play an important role in the survival of the individual and of species. This is realized by the regulation of salt and sugar homeostasis by mineralocorticosteroids and glucocorticosteroids and of reproductive processes by sex hormones.
Hofland, J., de Jong, F. H.
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Molecular Cloning of Steroid Hydroxylases
Endocrine Research, 1984Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
Maliyakal E John +2 more
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[29] Adrenal steroid hydroxylases
Publisher Summary This chapter discusses the general procedures used in the laboratories for separating mitochondria and microsomes from the adrenal cortex of steers and for preparing a reaction medium for the hydroxylase assays. The chapter summarizes the most common steroid assays. Modifications of these procedures are pointed out on the individual
Otto Rosenthal, Shakunthala Narasimhulu
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Effect of sulfonated steroids on steroidogenic cytochrome P450-dependent steroid hydroxylases
Journal of Steroid Biochemistry and Molecular Biology, 2018In the last decades, sulfonated steroids evolved from inactive metabolites intended for excretion to highly relevant compounds involved in many physiological processes. Investigations of the impact of sulfonated steroids on the steroid hormone biosynthesis revealed that, on the one hand, these can serve as substrate for steroidogenic cytochromes P450 ...
Rita Bernhardt
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Role of microsomal steroid hydroxylases in Δ7-steroid biosynthesis
Biochemistry (Moscow), 2013CYP17 (steroid 17α-hydroxylase/17,20-lyase) is a key enzyme in steroid hormone biosynthesis. It catalyzes two independent reactions at the same active center and has a unique ability to differentiate Δ(4)-steroids and Δ(5)-steroids in the 17,20-lyase reaction.
T A, Sushko +3 more
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Enzyme-activated inhibitors of steroidal hydroxylases
The Journal of Steroid Biochemistry and Molecular Biology, 1995Cytochrome P450 monooxygenases (CYP450) of the steroid biosynthetic pathways are highly substrate specific in comparison to the variable specificities of hepatic CYP450 enzymes. Both groups of enzymes catalyze the reductive cleavage of molecular oxygen with transfer of oxygen to the substrate to form hydroxylated derivatives.
J O, Johnston, C L, Wright, G W, Holbert
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Regulation of 21-Hydroxylase activity by steroids
Endocrine Research, 1995In this study, we investigated the effect of steroids on guinea pig and bovine adrenal steroidogenesis, especially 21-hydroxylase activity. Analysis of guinea pig adrenal steroids indicated the presence of high concentrations of androstenedione in the guinea pig adrenal; furthermore, in vitro studies using guinea pig adrenal cortex cells in primary ...
A, Bélanger +4 more
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