Results 161 to 170 of about 3,508 (215)

Rapid degradation of steroid sulfatase in multiple sulfatase deficiency

Biochemical and Biophysical Research Communications, 1986
Pulse labeling followed by SDS-PAGE electrophoresis of immunoprecipitated [35S]methionine-labeled steroid sulfatase (STS) gave a single band of molecular weight 65,000 daltons. After a chase period of 18 hours the material appeared as molecular weight approximately 64,000.
A L, Horwitz, L, Warshawsky, J, King, G, Burns   +3 more
openaire   +2 more sources

Microsomal steroid sulfatase: interactions with cytosolic steroid sulfotransferases

Chemico-Biological Interactions, 1998
Net sulfation of 4-methylumbelliferone in intact hepatocytes is regulated, in part, by substrate cycling between sulfotransferases (SULT) and arylsulfatases (ARS). Thus, ARS have the potential to influence rates of net sulfate conjugation of a variety of compounds in intact cells via interaction with SULT.
F C, Kauffman, S, Sharp, B B, Allan, A, Burchell, M W, Coughtrie   +4 more
openaire   +2 more sources

Genetic heterogeneity of steroid sulfatase deficiency revealed with cDNA for human steroid sulfatase

Biochemical and Biophysical Research Communications, 1987
Three cDNA clones with inserts of 1.2-1.6 kb that reacted both with antibodies and oligonucleotides specific for steroid sulfatase were isolated from a human placental library in lambda gt11. The 5'-end of one of the inserts, STS-3, was sequenced and colinearity with the amino acid sequence of 3 peptides of steroid sulfatase encompassing 64 amino acids
J T, Conary, G, Lorkowski, B, Schmidt, R, Pohlmann, G, Nagel, H E, Meyer, C, Krentler, J, Cully, A, Hasilik, K, von Figura   +9 more
openaire   +2 more sources

Steroid Sulfatase Activity in Osteoblast Cells

Biochemical and Biophysical Research Communications, 1997
We have demonstrated steroid sulfatase activity in osteoblast cells and characteristics of the enzyme were also investigated. Cell free homogenate of rat osteoblast cell line, UMR106-01 and human osteoblast cell lines, MG-63, HOS were incubated with [3H] dehydroepiandrosterone-sulfate (DHEA-sulfate) or [3H] estrone-sulfate (E1-sulfate).
H, Fujikawa, F, Okura, Y, Kuwano, A, Sekizawa, H, Chiba, K, Shimodaira, H, Saito, T, Yanaihara   +7 more
openaire   +2 more sources

Transcriptional control of human steroid sulfatase

The Journal of Steroid Biochemistry and Molecular Biology, 2009
Steroid sulfatase (STS) is a membrane-bound microsomal enzyme that hydrolyzes various alkyl and aryl steroid sulfates, leading to the in situ formation of biologically active hormones. The entire human STS gene spans over approximately 200kbp of which the first 100kbp include the regulatory region, while the STS-coding region is located downstream ...
NARDI, ALESSIA, POMARI, ELENA, ZAMBON D, BELVEDERE, PAOLA, COLOMBO, LORENZO, DALLA VALLE, LUISA   +5 more
openaire   +2 more sources

Steroid Sulfatase: Molecular Biology, Regulation, and Inhibition [PDF]

Endocrine Reviews, 2005
Steroid sulfatase (STS) is responsible for the hydrolysis of aryl and alkyl steroid sulfates and therefore has a pivotal role in regulating the formation of biologically active steroids. The enzyme is widely distributed throughout the body, and its action is implicated in physiological processes and pathological conditions. The crystal structure of the
M J, Reed, A, Purohit, L W L, Woo, S P, Newman, B V L, Potter   +4 more
exaly   +3 more sources

Steroid Sulfatase Activity in Human Leukocytes

Gynecologic and Obstetric Investigation, 1994
To study steroid sulfatase activity in women in the field of obstetrics and gynecology, especially to differentiate carrier women with steroid sulfatase deficiency (recessive X-linked ichthyosis, RXLI) from normal women, steroid sulfatase activity was assayed in peripheral blood leukocytes from normal nonpregnant women, pregnant women, patients with ...
I, Miyakawa, Y, Kawano, K, Taniyama, N, Mori   +3 more
openaire   +2 more sources

Role of steroid sulfatase in steroid homeostasis and characterization of the sulfated steroid pathway: Evidence from steroid sulfatase deficiency

Molecular and Cellular Endocrinology, 2016
The impact of steroid sulfatase (STS) activity in the circulating levels of both sulfated and unconjugated steroids is only partially known. In addition, the sulfated steroid pathway, a parallel pathway to the one for unconjugated steroids, which uses the same enzymes, has never been characterized in detail before.
Alberto Sánchez-Guijo, Jens Neunzig, Adrian Gerber, Vinzenz Oji, Michaela F. Hartmann, Hans-Christian Schuppe, Heiko Traupe, Rita Bernhardt, Stefan A. Wudy   +8 more
openaire   +2 more sources

Steroidal oxathiazine inhibitors of estrone sulfatase

Steroids, 2003
The presence of estrone sulfatase in breast tumors and the high levels of circulating estrone sulfate may contribute the major portion of estrogen synthesized locally in breast tissues through conversion of estrone sulfate to estrone by the enzyme. Using inhibitors of estrone sulfatase for the treatment of estrogen-dependent (estrogen receptor positive,
Richard H, Peters, Wan Ru, Chao, Barbara, Sato, Kazuhiko, Shigeno, Nurulain T, Zaveri, Masato, Tanabe   +5 more
openaire   +2 more sources

First Dual Aromatase-Steroid Sulfatase Inhibitors

Journal of Medicinal Chemistry, 2003
Aromatase inhibitors in clinical use block the biosynthesis of estrogens. Hydrolysis of estrone 3-sulfate by steroid sulfatase is an important additional source of tumor estrogen, and blockade of both enzymes should provide a more effective endocrine therapy.
Woo, L.W.L., Sutcliffe, O.B., Bubert, C., Grasso, A., Chander, S.K., Purohit, A., Reed, M.J., Potter, B.V.L.   +7 more
openaire   +3 more sources