Results 211 to 220 of about 452,915 (259)

Role of steroid sulfatase in steroid homeostasis and characterization of the sulfated steroid pathway: Evidence from steroid sulfatase deficiency

Molecular and Cellular Endocrinology, 2016
The impact of steroid sulfatase (STS) activity in the circulating levels of both sulfated and unconjugated steroids is only partially known. In addition, the sulfated steroid pathway, a parallel pathway to the one for unconjugated steroids, which uses the same enzymes, has never been characterized in detail before.
Alberto Sánchez-Guijo, Jens Neunzig, Adrian Gerber, Vinzenz Oji, Michaela F. Hartmann, Hans-Christian Schuppe, Heiko Traupe, Rita Bernhardt, Stefan A. Wudy   +8 more
openaire   +3 more sources

Genetic heterogeneity of steroid sulfatase deficiency revealed with cDNA for human steroid sulfatase

Biochemical and Biophysical Research Communications, 1987
Three cDNA clones with inserts of 1.2-1.6 kb that reacted both with antibodies and oligonucleotides specific for steroid sulfatase were isolated from a human placental library in lambda gt11. The 5'-end of one of the inserts, STS-3, was sequenced and colinearity with the amino acid sequence of 3 peptides of steroid sulfatase encompassing 64 amino acids
Bernhard Schmidt, Helmut E. Meyer, Gerhard Lorkowski, Jon T. Conary, Andrej Hasilik, Jan Cully, Gerhard Nagel, C. Krentler, Regina Pohlmann, Kurt von Figura   +9 more
openaire   +3 more sources

Rapid degradation of steroid sulfatase in multiple sulfatase deficiency

Biochemical and Biophysical Research Communications, 1986
Pulse labeling followed by SDS-PAGE electrophoresis of immunoprecipitated [35S]methionine-labeled steroid sulfatase (STS) gave a single band of molecular weight 65,000 daltons. After a chase period of 18 hours the material appeared as molecular weight approximately 64,000.
Allen L. Horwitz, Larry Warshawsky, Gary Burns, Judy King   +3 more
openaire   +3 more sources

Dual Aromatase−Steroid Sulfatase Inhibitors

Journal of Medicinal Chemistry, 2007
By introducting the steroid sulfatase inhibitory pharmacophore into aromatase inhibitor 1 (YM511), two series of single agent dual aromatase-sulfatase inhibitors (DASIs) were generated. The best DASIs in vitro (JEG-3 cells) are 5, (IC50(aromatase) = 0.82 nM; IC50(sulfatase) = 39 nM), and 14, (IC50(aromatase) = 0.77 nM; IC50(sulfatase) = 590 nM).
Woo, L.W.L., Bubert, C., Sutcliffe, O.B., Smith, A., Chander, S.K., Mahon, M.F., Purohit, A., Reed, M.J., Potter, B.V.L.   +8 more
openaire   +4 more sources

Transcriptional control of human steroid sulfatase

The Journal of Steroid Biochemistry and Molecular Biology, 2009
Steroid sulfatase (STS) is a membrane-bound microsomal enzyme that hydrolyzes various alkyl and aryl steroid sulfates, leading to the in situ formation of biologically active hormones. The entire human STS gene spans over approximately 200kbp of which the first 100kbp include the regulatory region, while the STS-coding region is located downstream ...
NARDI, ALESSIA, POMARI, ELENA, ZAMBON D, BELVEDERE, PAOLA, COLOMBO, LORENZO, DALLA VALLE, LUISA   +5 more
openaire   +3 more sources

Testicular steroid sulfatase

Steroids, 1969
Abstract Kinetic studies of the cleavage of dehydroepiandrosterone-sulfate (1) and androstenediol-3-sulfate by a particulate enzyme preparation from a rat testicular microsomal fraction gave Km values of 2.04 × 10−6M for DHA-S and 0.935 × 10−6M for androstenediol-3-sulfate with identical Vmax values. Inhibition studies with equimolar concentrations
Payne, Anita H., Mason, Merle, Jaffe, Robert B.   +2 more
openaire   +3 more sources

Achieving the ratiometric imaging of steroid sulfatase in living cells and tissues with a two-photon fluorescent probe.

Chemical Communications, 2020
Herein, a novel two-photon ratiometric fluorescence assay was proposed for monitoring endogenous steroid sulfatase (STS) activity, which could be applied for the ratiometric imaging of STS activity in the endoplasmic reticulum of living cells and tissues
Wei Li, Shulu Yin, Xiangyang Gong, Wang Xu, Ronghua Yang, Yichao Wan, Lin Yuan, Xiaobing Zhang   +7 more
semanticscholar   +1 more source

Inhibition of steroid sulfatase activity by danazol

Acta Obstetricia et Gynecologica Scandinavica, 1984
Abstract. The hydrolysis of dehydroepiandrosterone sulfate (DHAS) by human liver cells in culture and the hydrolysis of and formation of estradiol‐17β (E2) from estrone sulfate PIS) by human breast tumor preparations in vitro were studies in the presence and absence of danazol.
Anton Döberl, Anton Döberl, Gunnar Rannevik, Gunnar Rannevik, Kjell Carlström, Kjell Carlström, Nils Wilking, Nils Wilking, Åke Pousette, Åke Pousette   +9 more
openaire   +2 more sources

Garlic (Allium sativum L.) Organosulfur Compounds Inhibit Breast Cancer Cell Proliferation by Decreasing Steroid Sulfatase Levels

Anticancer Research
Background/Aim: Breast cancer is mostly affected by estrogen, which promotes proliferation, tumorigenesis, and cancer progression. Estrogen sulfotransferase (SULT1E1) catalyzes sulfation to inactivate estrogens, whereas steroid sulfatase (STS) catalyzes ...
Akira Sato, Ayano Yabuki, Genta Sato, Hina Nemoto, Yuta Ogawa, Makoto Ohira   +5 more
semanticscholar   +1 more source

Steroid Sulfatase Activity in Osteoblast Cells

Biochemical and Biophysical Research Communications, 1997
We have demonstrated steroid sulfatase activity in osteoblast cells and characteristics of the enzyme were also investigated. Cell free homogenate of rat osteoblast cell line, UMR106-01 and human osteoblast cell lines, MG-63, HOS were incubated with [3H] dehydroepiandrosterone-sulfate (DHEA-sulfate) or [3H] estrone-sulfate (E1-sulfate).
Hiroshi Saito, Kazuhisa Shimodaira, Akihiko Sekizawa, Takumi Yanaihara, Hiroshi Fujikawa, Yuzuru Kuwano, Fumiya Okura, Hiroshi Chiba   +7 more
openaire   +3 more sources