Results 91 to 100 of about 2,510,408 (298)
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
FACTORS INFLUENCING OPTIMAL STOCKING RATES FROM A TENANT PERSPECTIVE [PDF]
The terms of grazing lease contracts potentially influence the tenants incentive to preserve the vegetation resource. Annual stocking rate decisions dictate the degree of overgrazing, which can be cumulative over long periods of time.
Langemeier, Michael R. +3 more
core
, 1983 Recorded oral history of Iris Stocking and her recollections of Monroe County ...
Stocking, Iris
core +1 more source
Influence of stocking density on weight gain and behavior of feedlot lambs
Small Ruminant Research, 2013 AbstractThe aim of this study was to verify the influence of the animal density on the weight gain and behavior of confined lambs. 86 animals were confined after weaning in 23 pens of two lambs each (double pens) and four pens of ten animals each (collective pens).
Leme, Thays Mayra da Cunha +4 more
openaire +3 more sources
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Determination of the best stocking density for rearing Huso huso [PDF]
, 2006 Effects of stocking density on growth, food conversion ratio, specific growth rate, percentage of body weight increase in Huso huso were studied in two phases. Juveniles below one year old with an average weight of 92.09±1.72 grams and stocking densities
Aghtoman, V. +3 more
core +1 more source
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Lake Michigan Salmonid Stocking Costs in Wisconsin [PDF]
The costs of stocking salmonids in the Wisconsin waters of Lake Michigan are estimated for 1985. Estimation procedures and costs per stocked and captured fish are presented. Fingerlings of a species were found in most cases to cost more per captured fish
Ives, David B. +2 more
core
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source