Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics
Biotechnology and Bioengineering, EarlyView.ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu +4 more
wiley +1 more source
Multiple independent genetic code reassignments of the UAG stop codon in phyllopharyngean ciliates. [PDF]
PLoS GenetMcGowan J +3 more
europepmc +2 more sources
Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery. [PDF]
Cell Rep Methods, 2023 Meineke B +7 more
europepmc +1 more source
Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5
Biotechnology and Bioengineering, EarlyView.ABSTRACT Interspecies cross‐reactive protein therapeutics that target conserved epitopes across species are critical for translational research. The present study showcases the engineering of an affibody molecule, originally discovered for binding to human death receptor 5 (hDR5) with 94 nM affinity, to simultaneously acquire cross‐reactivity to murine
Tse‐Han Kuo +3 more
wiley +1 more source
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression. [PDF]
Proc Natl Acad Sci U S A, 2023 Jiang HK +5 more
europepmc +1 more source
Genetic code expansion for chemosensing and chemical actuation of biological functions
Bulletin of the Korean Chemical Society, EarlyView.Genetic code expansion (GCE) broadens the chemical repertoire for precise control of protein function in biological systems. This review highlights GCE‐based sensing and actuating systems that operate through selective interactions between noncanonical amino acids and chemical triggers.
Jieun Bae, Dong‐Hyun Kim, Minseob Koh
wiley +1 more source
The power of many: when genetics met yeasts and high‐throughput
Biological Reviews, EarlyView.ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
Cysteine tRNA acts as a stop codon readthrough-inducing tRNA in the human HEK293T cell line. [PDF]
RNA, 2023 Valášek LS +3 more
europepmc +1 more source
Endless possibilities: translation termination and stop codon recognition [PDF]
Microbiology, 2001 Gwyneth, Bertram +4 more
openaire +2 more sources