Results 61 to 70 of about 124,142 (266)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Subgeometric Ergodicity under Random-Time State-Dependent Drift Conditions
Journal of Probability and Statistics, 2014 Motivated by possible applications of Lyapunov techniques in the stability of stochastic networks, subgeometric ergodicity of Markov chains is investigated.
Mokaedi V. Lekgari
doaj +1 more source
Inhibitory control processes and the strategies that support them during hand and eye movements
Frontiers in Psychology, 2016 Background and Aims: Adaptive behavior depends on the ability to voluntarily suppress context-inappropriate behaviors, a process referred to as response inhibition. Stop Signal tests (SSTs) are the most frequently studied paradigm used to assess response
Lauren Michelle Schmitt +3 more
doaj +1 more source
On simple representations of stopping times and stopping time sigma-algebras [PDF]
Statistics & Probability Letters, 2013 Abstract There exists a simple, didactically useful one-to-one relationship between stopping times and adapted cadlag (RCLL) processes that are non-increasing and take the values 0 and 1 only. As a consequence, stopping times are always hitting times.
openaire +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Braking bad: stopping translation in hard times. [PDF]
PLoS Biology, 2014 Roland G Roberts
doaj +1 more source
The Dynamical Interaction between Low-mass Planets and Dust Coagulation
The Astrophysical Journal LettersWe investigate the impact of a low-mass planet on dust coagulation, and its consequent feedback on planetary migration, using a linear analysis of the coupled dust–gas hydrodynamic equations.
Qiang Hou, Cong Yu, Shu-ichiro Inutsuka
doaj +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source