Results 201 to 210 of about 2,765 (281)

The EU Strategic Compass: A Blueprint for a European Defensive and Securitisation Policy

, 2022
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Clinical trial protocol of PODRACING: A randomized controlled trial evaluating 3D perfusion zone models for selective arterial clamping during robot-assisted partial nephrectomy. [PDF]

BJUI Compass
Vangeneugden J, Vermijs S, De Backer P, Berquin C, Lumen N, De Kuyper P, Raskin Y, Bynens B, Baekelandt F, Ghysel C, Peeren F, De Groote R, De Naeyer G, Mottrie A, De Buyser S, De Visschere P, Debbaut C, Decaestecker K, Van Praet C.   +18 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Systemic Health System Measurement Framework: An Approach Based on the Unified Care Model for Whole Systems Transformation. [PDF]

Healthcare (Basel)
Lim T, Hu Y, Lee AWY, Tan JK, Ngoi QY, Liu N, Tay ACL, Lee JGJ, Ng YF.   +8 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Health promotion at the crossroads: putting civil society in the driver's seat. [PDF]

Health Promot Int
Shiroya V, Bost T, Kadasia B, Sozanski G.   +3 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

The Compass of Commitment: Control Mechanisms Underpinning the Sense of Individual and Joint Commitment. [PDF]

Cogn Sci
Kaufmann A, Fanghella M, Michael J.
europepmc   +1 more source