Improvement of Mouse Spermatozoa Freezing at ‐80°C With Ascorbic Acid 2‐Glucoside at Thawing Phase
ABSTRACT Background Sperm cryopreservation is the most common method to maintain a great number of mutant mouse lines. However, the use of liquid nitrogen (LN2) for freezing presents considerable problems in terms of cost, safety, and accessibility. For this reason, the storage of semen samples at ‐80° in ultra‐freezers has been increasingly used in ...
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