Results 151 to 160 of about 726,711 (265)

Structural Characterization and Anti-Alzheimer's Disease Effect of Polysaccharides From Stellariae Radix. [PDF]

Food Sci Nutr
Sun M, He J, Song H, Ma Z, Zhang X, Li J, Yao Y.   +6 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Heterologous expression and structural characterization of polyamide 4-degrading enzyme from a soil bacterium. [PDF]

Front Microbiol
Saito Y, Sato S, Sasanami Y, Yamashita T, Yamada M.   +4 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Structural characterization of the human CC2D1A fragment associated with non-syndromic intellectual disability (NSID). [PDF]

Biosci Rep
Yeh YH, Lin MG, Sun XH, Shen YY, Ling P, Hsiao CD.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Structural characterization of the native oligomerization mode of MvaT proteins in <i>Pseudomonas</i>. [PDF]

Microbiol Spectr
Vasileva D, Suzuki-Minakuchi C, Arakawa T, Moriwaki Y, Yonezawa K, Shimizu N, Fujimoto Z, Terada T, Okada K, Nojiri H.   +9 more
europepmc   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Structural characterization of the HDV virion and its ribonucleoprotein. [PDF]

Proc Natl Acad Sci U S A
Itskanov S, Ary B, Mehra U, Lew I, Novikov N, Schmitz U, Holdorf MM, Beran RK, Lansdon EB.   +8 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source