Results 81 to 90 of about 4,870 (264)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Énumération et structuration discursive
Dans cet article, la structure énumérative est envisagée, dans une perspective discursive, en tant que procédé d'organisation du texte constituant un tout fonctionnel.
Rebeyrolle Josette +1 more
doaj +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Design and analysis strategies for robust microbiome ageing research
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Digital transformation has reshaped media ecosystems by positioning YouTube as a dominant platform for producing, distributing, and consuming content within the logic of algorithmic capitalism.
Khoirul Hudah, Luxy Nabela Farez
doaj +1 more source
The article presents a functional-structural analysis of character patterns, integrating insights from psychoanalysis, body-oriented psychotherapy, and the Russian psychophysiological school. The author conceptualizes character as a multi-level dynamic system that combines cognitive, emotional, behavioral, and somatic components.
openaire +1 more source
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Local journalists’ response to foreign tourist misconduct in post-pandemic Bali
The emergence of Bali as a world tourist destination has been accompanied by a series of problems involving misconduct by foreign tourists. This paper examines how local journalists cope with these issues whilst managing professional ethics, political ...
Lestari Nurhajati +2 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source

