Results 141 to 150 of about 9,024,995 (297)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

Photodegradable antimicrobial agents: towards structure optimization. [PDF]

open access: yesRSC Adv, 2023
Souto de Aquino GA   +7 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Structure Optimization of Planar Nanoscale Vacuum Channel Transistor. [PDF]

open access: yesMicromachines (Basel), 2023
Xu J   +5 more
europepmc   +1 more source

Well-posedness and scalarization in vector optimization [PDF]

open access: yes
In this paper we study several existing notions of well-posedness for vector optimization problems. We distinguish them into two classes and we establish the hierarchical structure of their relationships.
Rocca Matteo   +2 more
core  

Multi-skill resource-constrained project scheduling problem: a case study in an open-pit mining process

open access: yes
In this article, a case study is analysed using an adaptation of a project scheduling problem with multitasking resource restrictions. The objective is to reduce the operational costs of an open-pit mining process that uses mobile surface equipment, by ...
Solano-Charris E.L.   +2 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Principles of Optimization for an External Thermo-Wall Structure

open access: yes
Optimization for an External Thermo-Walls ere ...
Krasņikovs, Andrejs   +1 more
core  

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

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