Results 171 to 180 of about 7,746 (198)

Antibody response to Shiga toxins Stx2 and Stx1 in children with enteropathic hemolytic-uremic syndrome. [PDF]

Journal of Clinical Microbiology, 2001
ABSTRACT A Western blot (immunoblot) assay (WBA) for the detection of immunoglobulin G antibodies to Shiga toxins Stx2 and Stx1 in sera from 110 patients with enteropathic hemolytic-uremic syndrome (53 culture confirmed to have Shiga toxin-producing Escherichia coli [STEC] infection) and 110 age-matched ...
Mohamed Karmali, Christoph Bobrowski, Martin Petric   +2 more
exaly   +3 more sources

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Activation of the caspase cascade during Stx1-induced apoptosis in Burkitt's lymphoma cells

Journal of Cellular Biochemistry, 2001
Shiga toxin 1 (Stx1) produced by Escherichia coli has been reported to induce apoptosis in many different cell types, including Burkitt's lymphoma (BL) cells. Since it has been established that the caspases play essential roles as the effector molecules in the apoptotic process in most cases, we examined the kinetics of caspase activation during the ...
Nobutaka Kiyokawa, Tetsuya Mori, Tae Takeda   +2 more
exaly   +3 more sources

Reconstitution of active recombinant Shiga toxin (Stx)1 from recombinant Stx1-A and Stx1-B subunits independently produced byE. coliclones

FEMS Microbiology Letters, 1999
Escherichia coli clones expressing recombinant Shiga toxin (Stx)1-A and recombinant Stx1-B subunits, were established. Culture supernatants of these clones were examined for inhibitory activity on in vitro protein synthesis using luciferase as a reporter enzyme.
T, Nishikawa, J, Fujii, S, Yoshida, T, Yutsudo   +3 more
openaire   +3 more sources

Neutralization of Shiga toxins Stx1, Stx2c, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. [PDF]

Infection and Immunity, 2001
ABSTRACT Strains of Escherichia coli producing Shiga toxins Stx1, Stx2, Stx2c, and Stx2d cause gastrointestinal disease and the hemolytic-uremic syndrome in humans. We have recently constructed a recombinant bacterium which displays globotriose (the receptor for these toxins) on its surface and adsorbs and
Renato Morona, James Paton, A W Paton
exaly   +4 more sources

Evaluation of the eazyplex® EHEC complete (Amplex) assay for the differentiation of stx1 and stx2 positivity in stool samples

Journal of Microbiological Methods, 2021
The performance of the eazyplex® EHEC complete (Amplex) for the detection of Shiga toxin genes in stool samples was evaluated. The assay performed well in distinguishing between stx1 and stx2 but suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing Escherichia coli infections.
Hannes Bjarki, Vigfússon, Theresa, Ennefors, Torbjörn, Kjerstadius, Martin, Sundqvist   +3 more
openaire   +2 more sources

Escherichia coli O157:H7 strains that express Shiga toxin (Stx) 2 alone are more neurotropic for gnotobiotic piglets than are isotypes producing only Stx1 or both Stx1 and Stx2.

Journal of Infectious Diseases, 2000
Infection with Escherichia coli O157:H7 can lead to hemolytic uremic syndrome (HUS) in some children. Epidemiologic data suggest that Shiga toxin (Stx) 2-producing strains are more frequently associated with HUS than are Stx1-producing strains. Less clear is whether strains that express Stx2 alone are more frequently associated with HUS than strains ...
Saul Tzipori, Arthur Donohue-Rolfe
exaly   +2 more sources

Genetic Variations in the stx1 and eae Genes of Bulgarian Milk Escherichia coli Isolates

Proceeding of the Bulgarian Academy of Sciences, 2013
Several types of Escherichia coli could cause intestinal and extra-intestinal diseases in humans and animals. Shiga toxin (Stx) is the virulence factor which causes haemorrhagic colitis. The eaeA gene gives the ability of the bacteria to induce lesions on the host colonic epithelial cells. The aim of this study is to compare the regions of subunit A of
Ivo Sirakov, Ralitsa Popova, Irina Alexandar, Atanaska Marinova-Petkova   +3 more
openaire   +1 more source

Characterization of the A subunit mutants of Stx1 and Stx2 in Saccharomyces cerevisiae

2009
Shiga toxins are a family of related toxins with two major groups, Stx1 and Stx2. The most common sources for Shiga toxin are the bacteria Shigella dysenteriae and the Shigatoxigenic group of Escherichia coli (STEC), which includes serotype O157:H7. Shiga-like toxin 1 and 2 (Stx1 and Stx2) are produced by some E. coli strains.
openaire   +1 more source

Detection and Evaluation of Escherichia coli O157:H7 and Shiga-toxin Genes stx1, stx2 in Cattle Meat Samples

Clinical Laboratory, 2018
Background: Screening of transmission routes and routine control of the food for foodborne-pathogens are vital in terms of public health. In this study, we aimed to investigate and evaluate the presence of E. coli O157:H7 strains and toxins in the cattle meat samples collected from different markets and butchers.
Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü, Savas Aslan, Altındiş, Mustafa, Recep Kara, Tayfur Demiray, Köroğlu, Mehmet, Altındiş, Mustafa, Demiray, Tayfur, Köroğlu, Mehmet   +8 more
openaire   +2 more sources

In Vitro Induction of Apoptosis in Human Lymphocytes By Stx1 From E. Coli O157:H7

Scientific Journal of King Faisal University: Basic and Applied Sciences
Background: Shiga toxin 1 (Stx1), produced by Escherichia coli O157:H7 and related strains, is a potent cytotoxin implicated in severe clinical conditions such as hemolytic uremic syndrome (HUS), acute kidney injury, and chronic urinary tract infections. While its mechanisms of protein synthesis inhibition and apoptosis induction are well characterized
Kadhm Hadi, Shayma’a Raisan
openaire   +1 more source