Results 61 to 70 of about 476 (134)
Background Bovine anaplasmosis, caused by the rickettsial tick-borne pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae), is vectored by Rhipicephalus (Boophilus)microplus in many tropical and subtropical regions of the world. A.
Jongejan Frans +7 more
doaj +1 more source
A) QRT-PCR analysis showing levels of O. turicata americanus 28S rRNA transcripts normalized to total RNA in samples generated from unfed and fed adult female ticks (A), unfed larvae, nymphs, and adults (B), salivary glands (C) and midgut (D) isolated ...
Girish Neelakanta (177788) +4 more
core +1 more source
subolesin gene sequence and structure are highly conserved.
(A) Percent identity matrix created by Clustal 2.1 for subolesin coding sequences in I. scapularis tick cell lines (1, IDE8; 3, ISE6) and tick strains (2, Wikel; 4, OSU; 5, NY; 6, RI1; 7, RI2).
Katherine M. Kocan (326058) +9 more
core +1 more source
(A) For gene knockdown by RNAi, ISE6 tick cells were incubated with subolesin or NF-kB (Relish) dsRNAs and compared to control cells incubated with the unrelated Rs86 dsRNA.
Katherine M. Kocan (326058) +9 more
core +1 more source
RNAi mediated silencing of subolesin causes significant downregulation of tick innate immune genes.
QRT-PCR analysis showing expression of O. turicata americanus toll (A) lp (B), fdp (C) cystatin (D) and mld (E) genes in mock-dsRNA or subolesin-dsRNA-treated fed adult female ticks.
Girish Neelakanta (177788) +4 more
core +1 more source
Primers and PCR conditions used for subolesin promoter analysis.
Primers and PCR conditions used for subolesin promoter analysis.
Katherine M. Kocan (326058) +9 more
core +1 more source
Primers and PCR conditions used for subolesin intron amplification.
Primers and PCR conditions used for subolesin intron amplification.
Katherine M. Kocan (326058) +9 more
core +1 more source
Evolution of the multifaceted eukaryotic
Background Akirins are nuclear proteins that form part of an innate immune response pathway conserved in Drosophila and mice. This studies aim was to characterise the evolution of akirin gene structure and protein function in the eukaryotes.
Johnston Ian A, Macqueen Daniel J
doaj +1 more source
Gene silencing of the tick protective antigens, Bm86, Bm91 and subolesin, in the one-host tick Boophilus microplus by RNA interference [PDF]
The use of RNA interference (RNAi) to assess gene function has been demonstrated in several three-host tick species but adaptation of RNAi to the one-host tick, Boophilus microplus, has not been reported.
Amar Taoufik +19 more
core +1 more source
Additional file 1: Table S1. Effect of rHlSu vaccination on control of H. longicornis infestation in rabbits. Figure S1. Evaluation of antibody titers.
Lee, Seung-Hun +9 more
openaire +1 more source

