Results 91 to 100 of about 797,048 (304)

Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU [PDF]

, 2002
Hsc66 and Hsc20 comprise a specialized chaperone system important for the assembly of iron-sulfur clusters in Escherchia coli. Only a single substrate, the Fe/S template protein IscU, has been identified for the Hsc66/Hsc20 system, but the mechanism by ...
Hoff, Kevin G., Silberg, Jonathan J., Ta, Dennis T., Tapley, Tim L., Vickery, Larry E.   +4 more
core   +1 more source

Substrate Specificity of Trypanothione Reductase [PDF]

European Journal of Biochemistry, 1997
Trypanothione redutase, one of the family of flavin‐dependent disulfide oxidoreductases, catalyses the reduction of trypanothione disulfide [N1,N8‐bis(glutathionyl)spermidine] and related glutathionyl‐polyamine disulfides. A series of subtly different, designed substrate analogues based on trypanothione were prepared by means of a solid‐phase approach ...
I R, Marsh, M, Bradley
openaire   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Block-based characterization of protease specificity from substrate sequence profile

BMC Bioinformatics, 2017
Background The mechanism of action of proteases has been widely studied based on substrate specificity. Prior research has been focused on the amino acids at a single amino acid site, but rarely on combinations of amino acids around the cleavage bond ...
Enfeng Qi, Dongyu Wang, Bo Gao, Yang Li, Guojun Li   +4 more
doaj   +1 more source

Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase

Nature Communications, 2020
Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans.
Ana García-García, Laura Ceballos-Laita, Sonia Serna, Raik Artschwager, Niels C. Reichardt, Francisco Corzana, Ramon Hurtado-Guerrero   +6 more
doaj   +1 more source

The purification and characterisation of novel dipeptidyl peptidase IV-like activity from bovine serum [PDF]

, 2004
The discovery of a potentially novel proline-specific peptidase from bovine serum is presented which is capable of cleaving the dipeptidyl peptidase IV (DPIV) substrate Gly-Pro-MCA.
Abbott, Birney, Cunningham, De Meester, De Meester, Duke-Cohan, Duke-Cohan, Duke-Cohan, Durinx, Gilmartin, Hegen, Hopsu-Havu, Huang, Iwaki-Egawa, Kin, Laemmli, Malik, Mentlein, Mentlein, Ohkubo, Ohnishi, Pineiro-Sanchez, Scanlan, Schon, Sedo, Sedo, Shibuya-Saruta, Smith, Stockel-Maschek, Underwood, Yaron, Yoshimoto   +31 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Substrate Specificity and Enzyme Recycling Using Chitosan Immobilized Laccase

Molecules, 2014
The immobilization of laccase (Aspergillus sp.) on chitosan by cross-linking and its application in bioconversion of phenolic compounds in batch reactors were studied.
Everton Skoronski, Mylena Fernandes, Maria de Lourdes Borba Magalhães, Gustavo Felippe da Silva, Jair Juarez João, Carlos Henrique Lemos Soares, Agenor Fúrigo Júnior   +6 more
doaj   +1 more source

A high-throughput screening method for determining the substrate scope of nitrilases [PDF]

, 2014
Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and
Black, Gary, Brown, Nicola, Perry, Justin, Randall, P. David, Turnbull, Graeme, Zhang, Meng   +5 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source