Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education
FEBS Open Bio, EarlyView.Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell +8 more
wiley +1 more source
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
FEBS Open Bio, EarlyView.Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
Abstract: The Public Face of Rhinoplasty: Impact on Perceived Attractiveness and Personality
Plastic and Reconstructive Surgery, Global Open, 2017 Stephen M. Lu, MD +7 more
doaj +1 more source
An Appearance of Succession Requires a Succession of Appearances
Philosophy and Phenomenological Research, 2012 A familiar slogan in the literature on temporal experience is that ‘a succession of appearances, in and of itself, does not amount to an experience of succession’. I show that we can distinguish between a strong and a weak sense of this slogan. I diagnose the strong interpretation of the slogan as requiring the support of an assumption I call the ...
openaire +2 more sources
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
FEBS Open Bio, EarlyView.Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Abstract: Umbilical Ablation During Abdominal Flap Harvest Decreases Donor Site Complications
Plastic and Reconstructive Surgery, Global Open, 2018 Mark Fisher, MD +6 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Abstract: The State of Plastic Surgery Education Outside of the Operating Room
Plastic and Reconstructive Surgery, Global Open, 2017 Mark Fisher, MD +5 more
doaj +1 more source