Results 171 to 180 of about 2,387 (211)

Methods for in situ visualization and assay of sulfurtransferases

Analytical Biochemistry, 1987
The dansyl derivative 5-dimethylamino-1-naphthalene thiosulfonate (DANTS) can serve as a sulfane sulfur-donor substrate for several of the sulfurtransferases, the reaction being dependent on the acceptor substrates supplied. Enzymatic cleavage of the sulfur-sulfur bond of DANTS releases the intrinsic fluorescence of the molecule, with an emission ...
John Westley, Bruce A. Aird, John Lane
openaire   +4 more sources

Chromogenic and fluorigenic substrates for sulfurtransferases

Methods in Enzymology, 1987
Publisher Summary This chapter discusses the chromogenic and fluorigenic substrates for sulfurtransferases. The enzymes rhodanese (thiosulfate : cyanide sulfurtransferase; thiosulfate sulfurtransferase) and thiosulfate reductase catalyze reactions in which a sulfane sulfur atom is transferred from inorganic thiosulfate anion or other suitable donor ...
John Westley, John Lane, Aiko M. Westley, Mary R. Burrous   +3 more
openaire   +4 more sources

Biosynthesis of Chuangxinmycin Featuring a Deubiquitinase‐like Sulfurtransferase

Angewandte Chemie, 2021
AbstractThe knowledge on sulfur incorporation mechanism involved in sulfur‐containing molecule biosynthesis remains limited. Chuangxinmycin is a sulfur‐containing antibiotic with a unique thiopyrano[4,3,2‐cd]indole (TPI) skeleton and selective inhibitory activity against bacterial tryptophanyl‐tRNA synthetase.
Xingwang Zhang, Xiaokun Xu, Cai You, Chaofan Yang, Jiawei Guo, Moli Sang, Ce Geng, Fangyuan Cheng, Lei Du, Yuemao Shen, Sheng Wang, Haidong Lan, Fan Yang, Yuezhong Li, Ya‐Jie Tang, Youming Zhang, Xiaoying Bian, Shengying Li, Wei Zhang   +18 more
openaire   +2 more sources

Rhodanese domain-containing sulfurtransferases: multifaceted proteins involved in sulfur trafficking in plants.

Journal of Experimental Botany, 2019
Sulfur is an essential element for the growth and development of plants that synthesize cysteine and methionine residues from the reductive assimilation of sulfate.
Benjamin Selles, Anna Moseler, N. Rouhier, Jérémy Couturier   +3 more
semanticscholar   +1 more source

A physical characterization of sulfane sulfurtransferase

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
The bacterial enzyme sulfane sulfurtransferase has been studied using spectroscopic techniques. The enzyme was characterized in terms of its near-UV absorption spectrum, molar ellipticity, intrinsic fluorescence spectra and the effects of general and ionic quenching reagents upon its fluorescence.
Paul M. Horowitz, Bruce A. Aird
openaire   +3 more sources

On the molecular weight of thiosulfate sulfurtransferase

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1975
Bovine liver thiosulfate sulfurtransferase (rhodanese) (EC 2.8.1.1) HAS BEEN REPORTED TO EXIST IN SOLUTION IN A RAPID, PH-dependent equilibrium between monomeric and dimeric forms of molecular weights 18 500 and 37 000 (Volini, M., DeToma, F. and Westley, J. (1967), J. Biol. Chem. 242, 5220).
Clare Woodward, Lynda M. Ellis
openaire   +3 more sources

Specificity studies of 3‐mercaptopyruvate sulfurtransferase

Journal of Biochemical Toxicology, 1995
Abstract3‐Mercaptopyruvate sulfurtransferase (E.C. 2.8.1.2; MST) is an enzyme believed to function in the endogenous cyanide (CN) detoxification system because it is capable of transferring sulfur from 3‐mercaptopyruvate (3‐MP) to CN, forming the less toxic thiocyanate (SCN). To date, 3‐MP is the only known sulfur‐donor substrate for MST.
Steven I. Baskin, Dale W. Porter
openaire   +3 more sources

Is Thiosulfate Sulfurtransferase the Colonic Sulfide Oxidase?

Digestive Diseases and Sciences, 2007
Neither sodium sulfide nor sodium thiosulfate is soluble in ethanol but NaHS.H20 is. We then removed insoluble material from our preparations by centrifugation and discarded it. The ethanol-soluble material was used in the experiments. In this later study we were able to show that removal of thiosulfate sulfurtransferase (TST), using a specific ...
Margaret C. Eggo, M. J. S. Langman, Rhian Warrack, Sundaram Ramasamy, S Singh   +4 more
openaire   +2 more sources

The presence of an inhibitor of tRNA sulfurtransferase in Morris hepatomas

Biochemical and Biophysical Research Communications, 1975
Summary A tRNA sulfurtransferase has been isolated from the 160, 000 g supernatant of Buffalo rat liver and a number of Morris hepatomas. This enzyme catalyzes the transfer of labeled sulfur from [36S]β-mercaptopyruvate to tRNA in the presence of ATP and magnesium ion.
Harold P. Morris, Mariel A. Harris, Ting-Wa Wong   +2 more
openaire   +3 more sources

Encapsulation of thiosulfate: Cyanide sulfurtransferase by mouse erythrocytes

Toxicology and Applied Pharmacology, 1986
Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including [14C]sucrose, [3H]inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate.
James L. Way, J. L. Way, D. Sylvester, Christian Sander, Lee E. Ray, Peter Leung   +5 more
openaire   +3 more sources