Results 91 to 100 of about 73,561 (294)
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Rheological behaviour and spectroscopic investigations of cerium-modified AlO(OH)colloidal suspensions [PDF]
, 2011 The rheological behaviour of aqueous suspensions of boehmite (AlO(OH)) modified with different Ce-salts (Ce(NO3)3, CeCl3, Ce(CH3COO)3 and Ce2(SO4)3) was investigated at a fixed Ce/Al molar ratio (0.05).
Bleta, Rudina +3 more
core +2 more sources
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Surface conditions of the Orgueil meteorite parent body as indicated by mineral associations [PDF]
Three periods of mineral formation from study of Orgueil ...
Bostrom, K., Fredriksson, K.
core +1 more source
Assessing the freshwater quality of a large-scale mining watershed : the need for integrated approaches [PDF]
, 2019 Water quality assessments provide essential information for protecting aquatic habitats and stakeholders downstream of mining sites. Moreover, mining companies must comply with environmental quality standards and include public participation in water ...
Arroyo, Nilton Deza +10 more
core +1 more source
FEBS Open Bio, EarlyView.We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
An ion probe study of the sulphur isotopic composition of Fe-Ni sulphides in CM carbonaceous chondrites [PDF]
, 2004 From the Introduction: The CM chondrites have endured variable degrees of aqueous alteration [1] which has changed their original mineralogy. A detailed study of the petrology and mineralogy of the sulphides in a suite of increasingly aqueously altered ...
Bullock, E. S. +4 more
core +1 more source
Mineralogy and distribution of critical elements in the Sn–W–Pb–Ag–Zn Huanuni deposit, Bolivia [PDF]
, 2019 The polymetallic Huanuni deposit, a world-class tin deposit, is part of the Bolivian tin belt. As a likely case for a “mesothermal” or transitional deposit between epithermal and porphyry Sn types (or shallow porphyry Sn), it represents a case that ...
Alfonso, Pura +12 more
core +2 more sources
TMC4 localizes to multiple taste cell types in the mouse taste papillae
FEBS Open Bio, EarlyView.Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source