Results 41 to 50 of about 2,084,287 (284)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Distance and T-square sampling for spatial measures of tree diversity
Distance sampling and its statistically improved variant, T-square sampling, are important sampling methods in plant ecology. They have often been applied in the context of plant density estimations and are comparatively easy to implement, since they ...
Arne Pommerening +2 more
doaj +1 more source
Assessment of forest resources for forest management.
The general requirements for forest information required in forest management include the availability of quantitative data concerning forest areas and timber volume, data describing that structure and quality of the forest by classes, data dealing with ...
Nyyssönen, Aarne
doaj +1 more source
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
2021 Limited access state numbered highways. As of 2020: Dec. 31.
Annual; Each v. reports data for the previous year; e.g. 1987 v. presents 1986 data.; Issued by: Road Inventory and Records Unit, ; Engineering Data and Inventory Section, -1982; Division of Planning Inventory and Data, 1983-1990; Planning Inventory and ...
core +1 more source
ABSTRACT Background Cognitive impairment is a common non‐motor symptom in Multiple Sclerosis (MS), negatively affecting autonomy and Quality of Life (QoL). Innovative rehabilitation strategies, such as semi‐immersive virtual reality (VR) and computerized cognitive training (CCT), may offer advantages over traditional cognitive rehabilitation (TCR ...
Maria Grazia Maggio +8 more
wiley +1 more source
The aim is to analyze the current state of mountain agriculture in a crisis of traditional agricultural civilization based on terraced agriculture.Results and discussion.
Z. M. Asadulaev, A. M. Musaev
doaj +1 more source
ABSTRACT Objective Isolated rapid eye movement sleep behavior disorder (iRBD) is a prodromal state for Lewy body disorders and exhibits biological heterogeneity that may influence clinical expression and progression. We examined clinical features in individuals with iRBD and biomarker‐defined synucleinopathy.
Daniel Weintraub +24 more
wiley +1 more source
The Development of E-Procurement System for an IT Consultant Company
The research objective was to identify, analyze, and design a procurement information system that was developed by an IT consultant company for their client companies.
Ong Alexander Ang, Arta Moro Sundjaja
doaj +1 more source

