Results 11 to 20 of about 1,958,325 (325)
Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation [PDF]
Methods in molecular biology, 2008 SUMO conjugation to protein substrates requires the concerted action of a dedicated E2 ubiquitin conjugation enzyme (Ubc9) and associated E3 ligases. Although Ubc9 can directly recognize and modify substrate lysine residues that occur within a consensus site for SUMO modification, E3 ligases can redirect specificity and enhance conjugation rates during
Ali A. Yunus, Christopher D. Lima
semanticscholar +5 more sources
Enzymes of the SUMO Modification Pathway Localize to Filaments of the Nuclear Pore Complex [PDF]
Molecular and Cellular Biology, 2002 SUMOs are small ubiquitin-related polypeptides that are reversibly conjugated to many nuclear proteins. Although the number of identified substrates has grown rapidly, relatively little is still understood about when, where, and why most proteins are modified by SUMO.
Hong Zhang +2 more
semanticscholar +6 more sources
SUMO conjugating enzyme: a vital player of SUMO pathway in plants
Physiology and Molecular Biology of Plants, 2021 Plants face numerous challenges such as biotic and abiotic stresses during their whole lifecycle. As they are sessile in nature, they ought to develop multiple ways to act during stressed conditions to maintain cellular homeostasis. Among various defense mechanisms, the small ubiquitin-like modifiers (SUMO) pathway is considered as the most important ...
Shantwana Ghimire +6 more
semanticscholar +6 more sources
Discovery and engineering of enhanced SUMO protease enzymes [PDF]
Journal of Biological Chemistry, 2018 Small ubiquitin-like modifier (SUMO) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a SUMO-specific protease is then used to remove SUMO from these proteins.
Yue-Ting K Lau +8 more
semanticscholar +3 more sources
Regulation of SUMOylation by Reversible Oxidation of SUMO Conjugating Enzymes [PDF]
Molecular Cell, 2006 Posttranslational modification with small ubiquitin-related modifier (SUMO) has emerged as a central regulatory mechanism of protein function. However, little is known about the regulation of sumoylation itself. It has been reported that it is increased after exposure to various stresses including strong oxidative stress. Conversely, we report that ROS
Guillaume Bossis, Frauke Melchior
semanticscholar +7 more sources
BCL11A is a SUMOylated protein and recruits SUMO‐conjugation enzymes in its nuclear body [PDF]
Genes to Cells, 2008 BCL11A/EVI9 is a zinc‐finger protein predominantly expressed in brain and hematopoietic cells. Previous studies show that BCL11A is involved in acute myelomonocytic leukemia and chronic lymphoid leukemia in mouse and human, respectively. Moreover, BCL11A is localized in the characteristic nuclear body in which BCL6 is co‐localized.
Takeshi Kuwata, Takuro Nakamura
semanticscholar +5 more sources
An Insight into the Factors Influencing Specificity of the SUMO System in Plants
Plants, 2020 Due to their sessile nature, plants are constantly subjected to various environmental stresses such as drought, salinity, and pathogen infections. Post-translational modifications (PTMs), like SUMOylation, play a vital role in the regulation of plant ...
Moumita Srivastava, Ari Sadanandom
doaj +2 more sources
International Journal of Genomics, 2020 SUMO-conjugating enzymes (SCE) and SUMO (Small Ubiquitin-Like Modifiers) genes are important components of SUMOylation. SCE has a crucial role during the SUMOylation process which acts as a catalyst to transfer SUMO to the target protein.
Shantwana Ghimire +6 more
doaj +2 more sources
Molecular mechanism of a covalent allosteric inhibitor of SUMO E1 activating enzyme [PDF]
Nature Communications, 2018 E1 enzymes activate ubiquitin (Ub) and ubiquitin-like modifiers (Ubls) in the first step of Ub/Ubl conjugation cascades and represent potential targets for therapeutic intervention in cancer and other life-threatening diseases.
Z. Lv +9 more
semanticscholar +5 more sources
The impact of dysregulation SUMOylation on prostate cancer [PDF]
Journal of Translational MedicineProstate cancer (PCa) remains one of the most common malignancies in men, with its development and progression being governed by complex molecular pathways.
Kailang Li +3 more
doaj +2 more sources