Results 81 to 90 of about 1,958,325 (325)

Assessing predictions of fitness effects of missense mutations in SUMO‐conjugating enzyme UBE2I [PDF]

Human Mutation, 2017
The exponential growth of genomic variants uncovered by next-generation sequencing necessitates efficient and accurate computational analyses to predict their functional effects. A number of computational methods have been developed for the task, but few unbiased comparisons of their performance are available.
Song Sun, Song Sun, Nick V. Grishin, Atina G. Cote, Atina G. Cote, Lisa N. Kinch, Jing Zhang, Frederick P. Roth, Qian Cong, Jochen Weile, Jochen Weile   +10 more
openaire   +4 more sources

High‐Throughput Synthesis and Screening of a Cyanimide Library Identifies Selective Inhibitors of ISG15‐Specific Protease mUSP18

Angewandte Chemie, EarlyView.
Deubiquitinases (DUBs) and Ub‐like proteases are attractive but challenging drug targets. We developed a high‐throughput synthesis method and prepared a 7536‐member cyanimide DUB‐targeted compound library in 1536‐well plates, using Echo acoustic dispensing. Screening yielded a selective, 35 nM inhibitor of mUSP18, the main ISG15 protease.
Raymond Kooij, Vito Pol, Jin Gan, Bjorn R. van Doodewaerd, Aysegul Sapmaz, Marta Campos Alonso, Günter Fritz, Klaus‐Peter Knobeloch, Paul P. Geurink   +8 more
wiley   +2 more sources

Knockdown of ubiquitin-like modifier-activating enzyme 2 promotes apoptosis of clear cell renal cell carcinoma cells

Cell Death and Disease, 2021
Small ubiquitin-related modifier (SUMO) proteins are involved in the development of tumors. Ubiquitin-like modifier-activating enzyme 2 (UBA2) is an important member of the SUMO modification system; however, its role in clear cell renal cell carcinoma ...
Guoxi Zhang, Junrong Zou, Jinglin Shi, Biao Qian, Kaiyang Qiu, Quanliang Liu, Tianpeng Xie, Zhihua He, Hui Xu, Yunfeng Liao, Yuting Wu, Yanmin Li, Guancheng Xiao, Yuanhu Yuan, Rihai Xiao, Gengqing Wu, Xiaofeng Zou   +16 more
doaj   +1 more source

The SUMO Conjugating Enzyme Ubc9 Is Required for Inducing and Maintaining Stem Cell Pluripotency [PDF]

Stem Cells, 2014
Abstract Sumoylation adds a small ubiquitin-like modifier (SUMO) polypeptide to the ε-amino group of a lysine residue. Reminiscent of ubiquitination, sumoylation is catalyzed by an enzymatic cascade composed of E1, E2, and E3. For sumoylation, this cascade uses Ubc9 (ubiquitin conjugating enzyme 9, now officially named ubiquitin ...
Mohammad Ghorbani, Soroush Tahmasebi, Xiang-Jiao Yang, Goran Gocevski, Paul Savage   +4 more
openaire   +3 more sources

Identification and developmental expression of Xenopus laevis SUMO proteases. [PDF]

PLoS ONE, 2009
SUMO proteins are small ubiquitin-related modifiers. All SUMOs are synthesized as propeptides that are post-translationally cleaved prior to conjugation.
Yonggang Wang, Debaditya Mukhopadhyay, Smita Mathew, Takashi Hasebe, Rachel A Heimeier, Yoshiaki Azuma, Nagamalleswari Kolli, Yun-Bo Shi, Keith D Wilkinson, Mary Dasso   +9 more
doaj   +1 more source

Ubiquitination accomplished: E1 and E2 enzymes were not necessary [PDF]

, 2016
Qiu et al. (2016) show that a mono-ADP-ribosyltransferase, SdeA, from Legionella pneumophila catalyzes ADP-ribosylation of ubiquitin, allowing SdeA to modify substrate with ubiquitin in the absence of E1 and E2 ...
Huang, Danny T., Nakasone, Mark
core   +1 more source

Evolution of molecular determinants for SUMO-activating enzyme subcellular localization in plants [PDF]

, 2020
AbstractPost-translational modification by Small Ubiquitin-related Modifier (SUMO) is an essential regulatory mechanism in eukaryotes. In the cell, SUMO conjugates are highly enriched in the nucleus and, consistently, SUMOylation machinery components are mainly nuclear.
Abraham Más, Laura Castaño-Miquel, Lorenzo Carretero-Paulet, Núria Colomé, Francesc Canals, L. Maria Lois   +5 more
openaire   +2 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

SUMO and cellular adaptive mechanisms

Experimental and Molecular Medicine, 2020
Stress response: how cells wrestle with SUMO disruption Cellular stress caused by disrupting attachment of the ubiquitous small ubiquitin-like modifier (SUMO) proteins, which are present in most organisms and regulate numerous DNA processes and stress ...
Hong-Yeoul Ryu, Seong Hoon Ahn, Mark Hochstrasser   +2 more
doaj   +1 more source

Biosynthesis of SUMOylated Proteins in Bacteria Using the Trypanosoma brucei Enzymatic System. [PDF]

PLoS ONE, 2015
Post-translational modification with the Small Ubiquitin-like Modifier (SUMO) is conserved in eukaryotic organisms and plays important regulatory roles in proteins affecting diverse cellular processes. In Trypanosoma brucei, member of one of the earliest
Paula Ana Iribarren, María Agustina Berazategui, Juan José Cazzulo, Vanina Eder Alvarez   +3 more
doaj   +1 more source