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Super-resolution microscopy demystified [PDF]
Nature Cell Biology, 2019 Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best ...
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2018
The term “super-resolution microscopy” (SRM) includes all the optical methodologies featuring spatial resolutions exceeding the diffraction limits of optical microscopes.
WANG LIN, MARTIN-FERNANDEZ MARISA
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The term “super-resolution microscopy” (SRM) includes all the optical methodologies featuring spatial resolutions exceeding the diffraction limits of optical microscopes.
WANG LIN, MARTIN-FERNANDEZ MARISA
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Introduction to super-resolution microscopy.
Microscopy (Oxford, England), 2014In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution.
Katsumasa Fujita, Nicholas Smith
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2022
Super resolution microscopy has become a staple in optical microscopy with applications ranging from imaging sub-cellular features to monitoring the stages of chemical reactions. In this chapter we present a brief background on the origin of the diffraction limit, a summary of the key techniques and their individual strengths and weaknesses.
Edward N. Ward, Robert Pal
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Super resolution microscopy has become a staple in optical microscopy with applications ranging from imaging sub-cellular features to monitoring the stages of chemical reactions. In this chapter we present a brief background on the origin of the diffraction limit, a summary of the key techniques and their individual strengths and weaknesses.
Edward N. Ward, Robert Pal
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2019
Optical microscopy is one of the most important scientific instruments in the history of mankind. It has revolutionized the field of life sciences and remains indispensable in many areas of scientific research. However, the resolution of the optical microscopy could not be enhanced infinitely through improving the amplification factor and eliminating ...
KANAROWSKI STAN, SHAFRAN EYAL
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Optical microscopy is one of the most important scientific instruments in the history of mankind. It has revolutionized the field of life sciences and remains indispensable in many areas of scientific research. However, the resolution of the optical microscopy could not be enhanced infinitely through improving the amplification factor and eliminating ...
KANAROWSKI STAN, SHAFRAN EYAL
+5 more sources
Nature Biotechnology
Abstract This chapter discusses the four main methods of achieving super-resolution, or microscope resolution that is better than what is attainable using diffraction-limited techniques and the Abbe or Rayleigh criteria. In order of discovery and increasing resolution the methods are pixel reassignment imaging, structured illumination ...
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Abstract This chapter discusses the four main methods of achieving super-resolution, or microscope resolution that is better than what is attainable using diffraction-limited techniques and the Abbe or Rayleigh criteria. In order of discovery and increasing resolution the methods are pixel reassignment imaging, structured illumination ...
+5 more sources
Multiview confocal super-resolution microscopy
Nature, 2021Confocal microscopy1 remains a major workhorse in biomedical optical microscopy owing to its reliability and flexibility in imaging various samples, but suffers from substantial point spread function anisotropy, diffraction-limited resolution, depth-dependent degradation in scattering samples and volumetric bleaching2.
Yicong Wu +26 more
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Fluorescent resolution target for super‐resolution microscopy
Journal of Microscopy, 2003SummaryHistorically, resolution in fluorescence optical microscopy has been limited by the Rayleigh criterion. Recently, however, several techniques have achieved resolution below that specified by the Rayleigh criterion. Among these are 4‐Pi confocal microscopy, harmonic excitation light‐microscopy, stimulated emission depletion microscopy, near‐field
P R H, Stark, L J, Rinko, D N, Larson
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Super-resolution microscopy writ large
Nature Biotechnology, 2016Improved expansion microscopy provides a super-resolution method that is accessible to any laboratory with a fluorescence microscope.
Peter Engerer +2 more
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Optical super-resolution microscopy in neurobiology
Current Opinion in Neurobiology, 2012Understanding the highly plastic nature of neurons requires the dynamic visualization of their molecular and cellular organization in a native context. However, due to the limited resolution of standard light microscopy, many of the structural specializations of neurons cannot be resolved.
Stephan J, Sigrist, Bernardo L, Sabatini
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