Results 21 to 30 of about 62,273 (254)

Super-resolution microscopy based on parallel detection [PDF]

Journal of Innovative Optical Health Sciences, 2019
Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C.
Zhimin Zhang, Shaocong Liu, Liang Xu, Yubing Han, Cuifang Kuang, Yong Liu, Xiang Hao, Hongqin Yang, Xu Liu   +8 more
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Application of convolutional neural networks towards nuclei segmentation in localization-based super-resolution fluorescence microscopy images

BMC Bioinformatics, 2021
Background Automated segmentation of nuclei in microscopic images has been conducted to enhance throughput in pathological diagnostics and biological research.
Christopher A. Mela, Yang Liu
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Structured illumination lensless digital holographic microscopy (SI-LDHM)

Frontiers in Physics
In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two ...
Juanjuan Zheng, Juanjuan Zheng, Juanjuan Zheng, Xuhong Guo, Xuhong Guo, Xuhong Guo, Ying Ma, Ying Ma, Ying Ma, Kai Wen, Kai Wen, Kai Wen, Sha An, Sha An, Sha An, Xiaofang Wang, Xiaofang Wang, Xiaofang Wang, Peng Gao, Peng Gao, Peng Gao, Jiaming Qian, Chao Zuo, Peng Gao   +23 more
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Super-resolution fluorescence microscopy studies of human immunodeficiency virus

Retrovirology, 2018
Super-resolution fluorescence microscopy combines the ability to observe biological processes beyond the diffraction limit of conventional light microscopy with all advantages of the fluorescence readout such as labelling specificity and non-invasive ...
Jakub Chojnacki, Christian Eggeling
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Adaptive optics in super-resolution microscopy

Biophysics Reports, 2021
Fluorescence microscopy has become a routine tool in biology for interrogating life activities with minimal perturbation. While the resolution of fluorescence microscopy is in theory governed only by the diffraction of light, the resolution obtainable in practice is also constrained by the presence of optical aberrations.
Wang, J, Zhang, Y
openaire   +3 more sources

Super Resolution Microscopy of SUMO Proteins in Neurons

Frontiers in Cellular Neuroscience, 2019
The ubiquitously expressed SUMO proteins regulate a plethora of cellular pathways and processes. While they have a predominantly nuclear localization, extranuclear roles of SUMO isoforms at the synapse have also been described, making SUMOylation one of ...
Luca Colnaghi, Luca Russo, Carmina Natale, Elena Restelli, Alfredo Cagnotto, Mario Salmona, Roberto Chiesa, Luana Fioriti   +7 more
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A guide to super-resolution fluorescence microscopy [PDF]

Journal of Cell Biology, 2010
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization
Schermelleh, Lothar, Heintzmann, Rainer, Leonhardt, Heinrich   +2 more
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Post-acquisition super resolution for cryo-electron microscopy

IUCrJ
Recently, reports have demonstrated achieving resolutions beyond the physical Nyquist limit using super resolution acquisition. Here, we demonstrate exceeding this limitation by pre-processing the raw micrograph movies from counting mode data that have ...
Raymond N. Burton Smith, Kazuyoshi Murata   +1 more
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Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved]

F1000Research, 2016
Dendritic spines and synapses are critical for neuronal communication, and they are perturbed in many neurological disorders; however, the study of these structures in living cells has been hindered by their small size.
Cristina M. Robinson, Mikin R. Patel, Donna J. Webb   +2 more
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Super-Resolution Structured Illumination Microscopy Reconstruction Using a Least-Squares Solver

Frontiers in Physics, 2020
Super-resolution microscopy enables images to be obtained at a resolution higher than that imposed by the diffraction limit of light. Structured illumination microscopy (SIM) is among the fastest super-resolution microscopy techniques currently in use ...
Jintao Luo, Chuankang Li, Qiulan Liu, Junling Wu, Haifeng Li, Cuifang Kuang, Cuifang Kuang, Cuifang Kuang, Xiang Hao, Xu Liu, Xu Liu, Xu Liu   +11 more
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