Results 21 to 30 of about 34,917 (151)

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli: Tuning inclusion body architecture and protein yield via dissolved oxygen control

open access: yesJournal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil   +7 more
wiley   +1 more source

Superación de los dualismos jurídicos en Kelsen

open access: yes, 1961
Superación de los dualismos jurídicos en ...
Gaviria Díaz, Carlos
core   +2 more sources

Nonpolar and Polar Lipids Composition in Recombinant Chinese Hamster Ovary Cells by Nile Red Staining Flow Cytometry

open access: yesEuropean Journal of Lipid Science and Technology, Volume 128, Issue 9, September 2026.
Cellular lipid composition is a dynamic process. Understanding it is important to improve recombinant protein production. Molecules with solvatochromic properties allow the determination of lipids of different polarities by flow cytometry and offer a useful method for monitoring lipids during different phases of cell growth in bioprocesses.
Francisco E. López‐Salas   +9 more
wiley   +1 more source

La superación teológica de la metafísica [PDF]

open access: yes, 2011
The thinking of Jean-Luc Marion has made possible a more resolute surpassing of metaphysics than the one tried by Heidegger. This theological surpassing, poinst out to a new way of thinking about god, disconnected from the question of being in the so ...
Restrepo, Carlos Enrique
core   +1 more source

Intercultural expectations and global ambitions: the spatial and temporal dimensions of Indigenous Amazonian adolescence Attentes interculturelles et ambitions globales: les dimensions spatiales et temporelles de l'adolescence chez les autochtones d'Amazonie

open access: yesJournal of the Royal Anthropological Institute, Volume 31, Issue S2, Page 31-49, September 2025.
This article explores the experiences of Indigenous Amazonian adolescents living and studying in a Peruvian metropolis, as they navigate the complex interplay between ethnic belonging, the older generation's ‘intercultural’ expectations, and ambitions for the future within a rapidly urbanizing and globalizing society.
Angela Giattino
wiley   +1 more source

El Laboratorio de Ecología en la UNCO: ¿un modelo “contra institucional” de gestión ? [PDF]

open access: yes, 2010
La investigación que basa esta ponencia busca comprender el modo en que los grupos académicos mantienen su producción y calidad a pesar de las fracturas históricas y condiciones institucionales desfavorables que los han impactado.
Ickowicz, Marcela   +2 more
core  

La dirección de la superación posdoctoral [PDF]

open access: yes, 2018
El artículo tiene como objetivo exponer el diseño de un modelo para la dirección de la superación posdoctoral que posibilite elevar la participación e integración de los recursos humanos en función de los procesos sustantivos que se desarrollan en las ...
Martín Garateix, Mario   +2 more
core   +1 more source

Improving the Efficiency of a Bioreactor Equipped With Mixed‐Flow Impellers

open access: yesChemical Engineering &Technology, Volume 48, Issue 7, July 2025.
The present work investigated the biological performance of mixed‐flow impellers. To the authors’ knowledge, this is the first experimental study on such impellers in a fermentation process using A. vinelandii, which was chosen as a microorganism due to its high oxygen demand.
David Posadas‐Navarro   +6 more
wiley   +1 more source

Reciprocal contributions: Indigenous perspectives and voices on marine‐coastal experiences in the channels of northern Patagonia, Chile

open access: yesPeople and Nature, Volume 7, Issue 5, Page 1086-1098, May 2025.
Abstract The recovery of marine ecosystems in areas heavily impacted by fishing and environmental exploitation depends, to some extent, on the sustainable management of resources and should be complemented by cultural traditions, practices and scientific knowledge.
Ricardo Alvarez   +9 more
wiley   +1 more source

Reliability and Validity Analysis of the AMAS‐Mobile for Assessing Anxiety in Mexican Higher Education Students

open access: yesHuman Behavior and Emerging Technologies, Volume 2025, Issue 1, 2025.
Anxiety is a prevalent issue among university students, with recent studies indicating that one in three students experiences it or another emotional disorder. To address this, the use of standardized scales has been proposed to assess anxiety in this population.
María Luisa González-Ramírez   +9 more
wiley   +1 more source

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