Early Learning Curve of a Novice Surgeon in Robot-Assisted Pulmonary Resection: Assessment Using the Robot-Assisted Thoracic Surgery Assessment Tool (RATSAT), Derived From the Video-Assisted Thoracoscopic Surgery Assessment Tool (VATSAT). [PDF]
Ishido K +8 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Satisfaction with academic supervision among nursing postgraduate students in China: a national multicenter cross-sectional study. [PDF]
Song J, Zhang L, Chen X, Wen H.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Potential agency and supervisor solutions to address burnout: Insights from clinicians in community mental health agencies across Washington State. [PDF]
Lu C +4 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Exploring Cultural Humility as a Framework for Supervising Second-Language Nursing Students Writing Their Bachelor's Thesis: A Qualitative Study. [PDF]
Roed MB +3 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
<i>Contextual factors influencing the performance of clinical preceptors: A scoping review protocol</i>. [PDF]
Jalife BP +4 more
europepmc +1 more source

