Results 141 to 150 of about 55,770 (219)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Interformat equivalence between paper and electronic administrations of the self-assessment anhedonia scale in a substance use disorder clinical sample. [PDF]

Front Psychol
Sanjuán Y, Estévez-Pérez V, Flórez G, Martínez-Montero M, Machado-Carrera I, Piñón-Blanco A, Iglesias-Rejas L, Rivera-Baltanás T, Sánchez-Luaces M, Campos-Pérez JA, López-García M, Olivares JM, Spuch C.   +12 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Predicting COPD outcomes with artificial intelligence and traditional statistics: common limitations in a heterogeneous condition. [PDF]

ERJ Open Res
Casal-Guisande M, Fernández-García A, Represas-Represas C, Fernández-Villar A.   +3 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

[Impact of the Healthcare Triad Model on Primary Care accessibility: a quasi-experimental before-and-after study]. [PDF]

Aten Primaria
Cañas Tornero F, Monge García MI, González Soria L, Rabadán Asencio A.   +3 more
europepmc   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Prise en charge de la multimorbidité cœur–cerveau : un guide de pratique clinique. [PDF]

CMAJ
Edwards JD, Li Z, McFarlane P, Rabi DM, Gilbert J, Bajaj HS, MacIntosh BJ, Bittman J, Feldman RD, Dresser G, Terenzi K, Swartz R, Gabor J, Pearson GJ, Selby P, Wharton S, Warburton DER, Pakhalé S, Styra R, Baker B, Tu K, Hawkins M, Stone JA, Vaillancourt T, Poon S, Virani SA, Jain R, Jones PH, Sandhu RK, Ganesh A, Andrade JG, Stern S, Habert J, Rivard L, Roumeliotis P, Udell JA, Campbell T, Bacon SL, Trudeau L, Keshavjee K, Pham T, Cheng G, Lewis KB, Maar M, Stacey D, Oldenburg B, Dhukai AR, Pasricha SV, Sherifali D, Racey M, Hua-Stewart D, Gravel CA, Lewis M, Mehta S, Hill MD, Haider N, Lindsay P, Liu P, Tobe SW.   +58 more
europepmc   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Myocardial protection by adenosine triphosphate-sensitive potassium channel opener diazoxide involves sulfonylurea receptor 2 subunit. [PDF]

JTCVS Open
Wang J, Bradshaw A, Tryon R, Pan R, Holmes SD, Nichols C, Lawton JS.   +6 more
europepmc   +1 more source