Results 111 to 120 of about 7,165,449 (296)

Metal-enhanced fluorescence: The role of quantum yield, Q, in enhanced fluorescence

, 2012
Metal-enhanced fluorescence has attracted enormous research and commercial interest in recent years, due to the ability to significantly enhance fluorescence signatures in the near-field as well as protect fluorophores against photobleaching.
Dragan, Anatoliy, Geddes, Chris
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Compact surface plasmon-enhanced fluorescence biochip

, 2013
A new concept of compact biochip for surface plasmon-enhanced fluorescence assays is reported. It takes advantage of the amplification of fluorescence signal through the coupling of fluorophore labels with confined and strongly enhanced field intensity ...
Dostálek, Jakub, Knoll, Wolfgang, Toma, Koji, Adam, Pavel, Vala, Milan, Homola, Jiří   +5 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Surface enhanced fluorescence by metallic nano-apertures associated with stair-gratings

Optics Express, 2016
Se proponen nanoaberturas metálicas asociadas con las rejillas de las escaleras para mejorar la fluorescencia de la superficie con una alta mejora de la excitación y un efecto de haz de emisión estrecho. Se utilizó el método de espectroscopia de correlación de fluorescencia para analizar la traza de fluorescencia y la mejora de la fluorescencia, y se ...
R. Yuanying Chou, Guantao Li, Yuqing Cheng, Yu He, Jingyi Zhao, Zhengmin Cao, Qihuang Gong, Guowei Lü   +7 more
openaire   +2 more sources

Synthesis of SiO2@Ag Nanocomposite for Investigating Metal-Enhanced Fluorescence and Surface-Enhanced Raman Spectroscopy

SiO2@Ag nanocomposite (NC) has been synthesized by the chemical reduction and Stӧber method for Metal-enhanced fluorescence (MEF) of Rhodmine 6G (R6G) and Surface-enhanced Raman spectroscopy (SERS) of Malachite green (MG).
Tran T.H., Nguyen T.-T., Bui Hong, Van, Sai C.D., Pham Van, Ben, Nguyen T.D., Nguyen T.H.G., Thai D.V., Tran Trong, Duc   +8 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Surface Enhanced Raman Scattering of a Lysosomal Fluorescent Dye

Analysis & Sensing
Knowledge about the molecular structure and interactions of intracellular dyes is important to understand their function and possible effects in the biological environment. Here we discuss vibrational spectra of LysoSensor (LSG), a fluorescent marker for the acidic lysosomal compartment in cells.
Cecilia Spedalieri, Janina Kneipp
openaire   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source