Results 101 to 110 of about 118,586 (322)

Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors

Advanced Science, EarlyView.
Hybrid FRET–BRET biosensors were developed to monitor RAF–KRAS interactions in living cells. These sensors enable real‐time visualization of interaction dynamics, quantitative spectral analysis, and validation of mutation‐selective inhibitors, providing a versatile platform for probing KRAS regulation and therapeutic responses. ABSTRACT The RAS–RAF–MEK–
Jeong‐Min Go, Dahee Lee, Minji Kim, Kiseok Han, Gyuho Choi, ChanHui Song, Sanghyun Ahn, Yerim Lee, Jinyoung Lee, Yingxiao Wang, Jung‐Soo Suh, Hwayoung Yun, Tae‐Jin Kim   +12 more
wiley   +1 more source

Targeting the GPX4–FUNDC1 Interaction with Magnesium Lithospermate B Attenuates Sepsis‐Associated Lung Injury

Advanced Science, EarlyView.
The diagram depicts the endothelial‐protective mechanism of magnesium lithospermate B (MLB) in sepsis‐associated lung injury. MLB binds GPX4 at Gly79, disrupts its interaction with FUNDC1, prevents mitophagy‐mediated GPX4 degradation, restores mitophagic flux, reduces ROS, and limits ferroptosis.
Zhixi Li, Chang Liu, Zhaoxue Ma, Dongyou Zheng, Renkai Wang, Yongjing Yu, Guangmin Chen, Chenglong Li, Yue Bu, Hang Cao, Bing Zhang   +10 more
wiley   +1 more source

Applications of Surface Plasmon Resonance (SPR) for the Characterization of Nanoparticles Developed for Biomedical Purposes

Italian National Conference on Sensors, 2012
Great interest is currently being devoted to the development of nanoparticles (NPs) for biomedical purposes, designed to improve the pharmacokinetic profile of their cargos (either imaging probes or drugs) and to enhance the specific targeting at the ...
Mara Canovi, Jacopo Lucchetti, M. Stravalaci, F. Re, D. Moscatelli, P. Bigini, M. Salmona, M. Gobbi   +7 more
semanticscholar   +1 more source

Potent and Selective IGF‐IIR‐Recruiting Bifunctional Molecules for Targeted Lysosomal Degradation of Extracellular and Membrane Proteins

Advanced Science, EarlyView.
Lysosome‐targeting chimeras (LYTACs) enable degradation of extracellular and membrane proteins via lysosomal trafficking. We report a novel IGF‐II mutant (Del1–7, Y27L) that selectively engages IGF‐IIR while avoiding IGF‐IR and IR‐A. mutIGF‐II–based LYTACs enhance target internalization and degradation and support a genetically encodable, all‐protein ...
Yuan Zhao, Yaxian Liao, Pengyun Li, Regina Stasser de Gonzalez, Xuankun Chen, Nicholas S. Nieto, Florence M. Brunel, Nick Cox, Joseph Stock, Matthew McHenry, Guangsen Fu, Penghsuan Huang, Wenxin Wu, Deqin Cai, Lingjun Li, Alexander N. Zaykov, Weiping Tang   +16 more
wiley   +1 more source

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

Semiconductor Physics, Quantum Electronics & Optoelectronics, 2016
The influence of the sensitive element fabrication technology and noise performance of surface plasmon resonance (SPR) immunosensor on sensitivity and stability of operation inherent to the “Plasmon” series instrument has been investigated to improve ...
R.V. Khrystosenko
doaj   +1 more source

Surface plasmon resonance study of the actin-myosin sarcomeric complex and tubulin dimers

, 2003
Biosensors based on the principle of surface plasmon resonance (SPR) detection were used to measure biomolecular interactions in sarcomeres and changes of the dielectric constant of tubulin samples with varying concentration.
Alexander A. Kolomenskii, Andreas Mershin, Brekhovskikh L. M., Brown J. A., Dimitri V. Nanopoulos, Earp R. L., Hans A. Schuessler, Mershin A., Raether H.   +8 more
core   +1 more source

Biomimetic Membrane Interface Technologies for Detection and Isolation of CTCs and EVs: Advances and Opportunities in Liquid Biopsy

Advanced Science, EarlyView.
Biomimetic membrane interface engineering constructs functionalized detection platforms by integrating natural cell membranes, synthetic lipids, or hybrid membranes. This strategy effectively reduces background interference and enables efficient target capture and analysis, showing broad applications in circulating tumor cell separation, extracellular ...
Duo Liu, Jie Yu, Jingxue Li, Yixue Chen, Mengle Peng, Yifan Xu, Yongjun Wu, Lihua Ding, Sitian He   +8 more
wiley   +1 more source

Precision‐Engineered Silver Single‐Atom Carbon Dot Nanozymes for Theranostic Management of Acute Kidney Injury

Advanced Science, EarlyView.
Mitochondria‐targeted silver single‐atom carbon dot (T‐AgSA‐CDs) nanozymes enable dual SOD/GPx‐like ROS scavenging, real‐time imaging, and therapeutic effects for acute kidney injury (AKI). Atomically dispersed Ag sites synergize with carbon dots to restore renal redox hemostasis, protect mitochondrial function, mitigate inflammation, facilitate post ...
Tianle Tang, Jun Zhang, Yan Wang, Guoping Chen, Kehui Yuan, Yuan He, Chenchen Li, Sumaira Hanif, Yang Yang, Yanli Wang, Pir Muhammad   +10 more
wiley   +1 more source

Parameter sensitivity analysis of surface plasmon resonance biosensor through numerical simulation

, 2010
Surface based analytical tools have gained more importance for rapid, sensitive and label-free monitoring of molecular recognition events. Surface plasmon resonance (SPR) has played a prominent role in real time monitoring of surface binding events.
Dai, Xiu J., Islam, Md. Saiful, Kouzani, Abbas Z., Michalski, Wojtek P.   +3 more
core   +1 more source

Unravelling Carbohydrate Interactions with Biosensors Using Surface Plasmon Resonance (SPR) Detection [PDF]

, 2001
Surface plasmon resonance (SPR) is an optical phenomenon occurring at a metal coated interface between two media of different refractive index, e.g., water and glass. Exploitation of this phenomenon for investigating biomolecular interactions has occurred since biomolecules can be attached to a chemically modified gold surface and that an interaction ...
Haseley, S.R., Kamerling, J.P., Vliegenthart, J.F.G.   +2 more
openaire   +3 more sources