Results 31 to 40 of about 191,290 (268)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

High-sensitivity detection of Mycobacterium tuberculosis DNA in tongue swab samples

Journal of Clinical Microbiology
Tongue swab (TS) sampling combined with quantitative PCR (qPCR) to detect Mycobacterium tuberculosis (MTB) DNA is a promising alternative to sputum testing for tuberculosis (TB) diagnosis.
Alaina M. Olson, Rachel C. Wood, Kris M. Weigel, Alexander J. Yan, Katherine A. Lochner, Rane B. Dragovich, Angelique K. Luabeya, Paul Yager, Mark Hatherill, Gerard A. Cangelosi   +9 more
doaj   +1 more source

Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds

Frontiers in Medicine, 2021
Chronic wounds are a prominent health concern affecting 0.2% of individuals in the Western population. Microbial colonization and the consequent infection contribute significantly to the healing process.
Aleksander Mahnic, Vesna Breznik, Maja Bombek Ihan, Maja Rupnik, Maja Rupnik   +4 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Elution of Artificial Sputum from Swab by Rotating Magnetic Field-Induced Mechanical Impingement

Applied Sciences, 2017
Cotton-tipped applicator swabs are used as a collection device for many biological samples and its complete elution is a desired step for clinical and forensic diagnostics.
Shubham Banik, James Mahony, P. Ravi Selvaganapathy   +2 more
doaj   +1 more source

New Matrixes to Diagnose Aethina tumida Presence at Apiary Level

Journal of Apicultural Science, 2021
Aethina tumida (Coleoptera: Nitidulidae - Small Hive Beetle - SHB), is a parasite of honey bee colonies that causes the notifiable disease called aethinosis. In 2014, SHB was detected in Southern Italy, where it is still present (Calabria region).
Cersini Antonella, Pietropaoli Marco, Pietrella Gabriele, Rivera-Gomis Jorge, Federico Giovanni, Tofani Silvia, Conti Raffaella, Rubino Roberta C., Di Ruggiero Camilla, Formato Giovanni   +9 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

An Italian Neurorehabilitation Hospital Facing the SARS-CoV-2 Pandemic: Data From 1207 Patients and Workers

Frontiers in Neurology, 2020
Objective: The aim of the present observational study is to report on the data from a large sample of inpatients, clinical staff and other workers at an Italian neurorehabilitation hospital dealing with SARS-CoV-2 infections, in order to analyze how it ...
Antonino Salvia, Giovanni Morone, Marco Iosa, Maria Pia Balice, Stefano Paolucci, Maria Grazia Grasso, Marco Traballesi, Ugo Nocentini, Rita Formisano, Marco Molinari, Angelo Rossini, Carlo Caltagirone   +11 more
doaj   +1 more source

SARS-CoV-2 detection in setting of viral swab scarcity: are MRSA swabs and viral swabs equivalent?

PLOS ONE, 2020
Abstract Background The global pandemic of Severe Acute Respiratory Syndrome-Related Coronavirus 2 (SARS-CoV2) has resulted in unprecedented challenges for healthcare systems. One barrier to widespread testing has been a paucity of traditional respiratory viral swab collection kits relative to the ...
Daniel G. Federman, Shaili Gupta, Gary Stack, Sheldon M. Campbell, David R. Peaper, Louise M. Dembry, Ann Fisher, Asim F. Tarabar, Michael Kozal, Christopher B. Ruser   +9 more
openaire   +4 more sources

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source