Results 231 to 240 of about 227,750 (314)

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Large Animal Models of Tricuspid Regurgitation: Relevance, Limitations, and Future Directions. [PDF]

J Cardiovasc Transl Res
Sukienik T, Nakamae K, Miyagi C, Yelisetty G, Hussaini SF, Watanabe T, Matsuda J, Yuhara S, Onohara D.   +8 more
europepmc   +1 more source

Screening Equine Skin and Mucous Membranes for Carriage of Coagulase‐Positive Staphylococci

Veterinary Dermatology, EarlyView.
ABSTRACT Background Commensal populations of coagulase‐positive Staphylococcus spp. (CoPS) in healthy horses are not well‐defined. Objective To determine the point prevalence, anatomical distribution, antimicrobial susceptibility and genomic characteristics of CoPS in healthy horses.
Christina M. Dierkes, Daniel O. Morris, Christine L. Cain, Laurel E. Redding, Maho Okumura, Alison Gore, Stephen D. Cole   +6 more
wiley   +1 more source

The livestock drinking water system as an active reservoir for antimicrobial resistance: A systematic review and one health gap analysis. [PDF]

PLoS One
Adhikari S, Khanal S, Adhikari A.
europepmc   +1 more source

One day of environment‐induced heat stress causes injury to the murine kidney

Experimental Physiology, EarlyView.
Abstract Environment‐induced heat stress (EIHS) results from sustained body temperature elevation owing to prolonged exposure to heat and humidity. We hypothesized that EIHS would cause kidney injury and cellular dysfunction. To test this hypothesis, female C57 mice were exposed to EIHS (n = 14; 37.6°C, 42.0% relative humidity) or thermoneutral (TN ...
Melissa Roths, Alyona Michael, Zachary J. Schlader, Joshua T. Selsby   +3 more
wiley   +1 more source

2D shear wave elastography in abdominal structures of nursery swine. [PDF]

BMC Vet Res
Giustina RD, Matos AS, da Motta B, Dos Santos KA, Cavagnari LSB, Comassetto F, da Cruz ICK, Carneiro RK.   +7 more
europepmc   +1 more source

Systemic pirfenidone treatment fails to mitigate fibrosis and compromises functional recovery in a porcine model of volumetric muscle loss

Experimental Physiology, EarlyView.
Abstract Repurposing clinically approved antifibrotic agents presents a potential near‐term therapeutic strategy to mitigate the extensive fibrotic scarring that follows severe volumetric muscle loss (VML) injury and inhibits functional recovery. We hypothesized that daily oral administration of pirfenidone, an FDA‐approved antifibrotic, would mitigate
Jessica M. Motherwell, Ondine Eken, Claudia E. Hernandez, Christopher L. Dearth, Stephen M. Goldman   +4 more
wiley   +1 more source

Presence of antimicrobial resistance genes in biofilms from swine drinking water pipes before and after treatment with peracetic acid. [PDF]

Front Microbiol
Doughan GE, Walthart BK, Schau CE, Skoland KJ, Mou KTY, Brown JT, Bonnema JL, Plummer PJ, Zhang D, Li G, Karriker LA.   +10 more
europepmc   +1 more source

An age‐associated decline in the role of the sarcoplasmic reticulum and associated calcium‐handling proteins sets the pace for sinoatrial node function

The Journal of Physiology, EarlyView.
Abstract figure legend Stable, responsive pacemaking in the sinoatrial node is driven by the activity of the funny current (membrane clock), interplay of calcium cycling and release from the sarcoendoplasmic reticulum with depolarising sodium‐calcium exchange current (calcium clock). With increasing age, key proteins associated with calcium cycling are
Sandra A. Jones, Fiona S. Godbeer, Matthew K. Lancaster   +2 more
wiley   +1 more source

Genomic characterization and recombination analysis of hepatitis E virus in humans and swine across Asia: implications for food safety. [PDF]

Front Microbiol
Liu D, He Z, Luo Q, Liu B, Zhang P, Chen J, Wang X, Wang G, Huang Y, Xiang H, Cai R.   +10 more
europepmc   +1 more source