Results 141 to 150 of about 895,397 (246)

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

IV-Oral Antibiotic Switch in Streptococcal and Enterococcal Endocarditis: Evaluating ESC Versus WikiGuideline Criteria. [PDF]

Pathogens
Mohamad A, van den Heuvel FMA, Kouijzer IJE.   +2 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Taking a Newer, Faster, Intranasal Route: A Narrative Review of Transitioning to a Less-Invasive Rescue Treatment for Seizure Clusters

Patient Preference and Adherence
Jurriaan M Peters,1 Danielle A Becker,2 Sunita N Misra,3 Enrique Carrazana,3,4 Adrian L Rabinowicz3 1Department of Neurology, Harvard Medical School and Boston Children’s Hospital, Boston, MA, USA; 2Department of Neurology, The Ohio State University ...
Peters JM, Becker DA, Misra SN, Carrazana E, Rabinowicz AL   +4 more
doaj  

A TSV-packaged SP6T RF MEMS switch with ultra-thin profile and suppressed substrate loss for DC to Ku band application. [PDF]

Microsyst Nanoeng
Zhang Y, Wei J, Liu H, Sun J, Wu Q, Li M, Liu Z.   +6 more
europepmc   +1 more source

Bakelite Light Switch Plate (Painted)

, 2010
Bakelite light switch plate, patterned inlay design (surrounding missing switch).. Maker: Duroid - from the The Betty Smithers Design Collection at Staffordshire University.
The Betty Smithers Design Collection at Staffordshire University, Staffordshire University   +1 more
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Affinity-Activated Protein Switches for Modular and Signal-ON Detection of Small Molecules. [PDF]

Anal Chem
Wu CC, Chen HM, Chang TJ, Weng LL, Wang YC, Chao KC, Lin JC, Jian BL, Chu LA, Tan KT.   +9 more
europepmc   +1 more source