Results 181 to 190 of about 391 (240)

Investigating the molecular mechanisms underlying the anti‐CRISPR function of AcrIIA13b protein

The FEBS Journal, EarlyView.
Anti‐CRISPR protein AcrIIA13b inhibits CRISPR–Cas9 function by preventing target DNA engagement. The Cas9–sgRNA ribonucleoprotein (RNP) normally recognizes the protospacer adjacent motif (PAM) duplex to initiate DNA cleavage. AcrIIA13b binding to the PAM‐interacting WED–PI domain blocks this recognition, leaving the target DNA intact.
So Yeon Lee, Hyun Ho Park
wiley   +1 more source

An Optimized Encryption Storage Scheme for Blockchain Data Based on Cold and Hot Blocks and Threshold Secret Sharing. [PDF]

Entropy (Basel)
Yang D, Tsai WT.
europepmc   +1 more source

Teleost‐specific ictacalcins exhibit similar structural organization, cation‐dependent activation, and transcriptional regulation as human S100 proteins

The FEBS Journal, EarlyView.
Calcium‐binding S100 proteins are important mediators of inflammation in humans. Distant members of this family have been recently discovered in teleost fish but their resemblance to human proteins has not been explored yet. This study demonstrates that zebrafish‐specific S100i1 and S100i2 (ictacalcins) possess identical structural organization and ...
Liz Hernández, Théo Paris, Maria Demou, Catherine Birck, Christina Begon‐Pescia, Juan Francisco Rodríguez Vidal, Sylwia D. Tyrkalska, Charlotte Bureau, Catherine Gonzalez, Juliette Gracia, Etienne Lelièvre, Victoriano Mulero, Mai Nguyen‐Chi, Laure Yatime   +13 more
wiley   +1 more source

Slide-block: End-to-end amplified security to improve DevOps resilience through pattern-based authentication. [PDF]

Heliyon
Sriraman G, R S.
europepmc   +1 more source

A Hanks‐type bacterial kinase, PknS, directly phosphorylates the alternative sigma factor EcfK to promote resistance to protist predation

The FEBS Journal, EarlyView.
The Xanthomonas citri Hanks‐type kinase PknS autophosphorylates and directly phosphorylates the alternative sigma factor EcfK at five residues. Besides the conserved residue T51 in the σ2 domain, phosphorylation of a residue in the linker between σ2 and σ4 is critical for EcfK activation by promoting its interaction with a positively charged pocket in ...
Lídia dos Passos Lima, Dev Sriranganadane, Daiane Laise da Silva, Natália C. Drebes Dörr, Enzo Breviglieri Sichi Mello, Caio Vinicius dos Reis, Rogério Ferreira Lourenço, José Felipe Teixeira da Silva Santos, Anita Salmazo, Brenno Wendler Miranda, Katlin B. Massirer, Rafael M. Couñago, Cristina E. Alvarez‐Martinez   +12 more
wiley   +1 more source

Comparative evaluation of noncanonical amino acids as site‐specific NMR probes for the complex of E. coli SSB with single‐stranded DNA without isotope labelling

The FEBS Journal, EarlyView.
Genetic encoding of noncanonical amino acids (ncAA) enables their site‐specific installation in proteins. This work compares the nuclear magnetic resonance (NMR) performance of different ncAAs in a large protein−DNA complex (~ 100 kDa) without isotope labelling. The ncAAs deliver readily detectable NMR signals in the complex between E.
Sreelakshmi Mekkattu Tharayil, Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Josemon George, Christoph Nitsche, Thomas Huber, Gottfried Otting   +7 more
wiley   +1 more source

Blockchain-based proxy re-encryption access control method for biological risk privacy protection of agricultural products. [PDF]

Sci Rep
Wang S, Luo N, Xing B, Sun Z, Zhang H, Sun C.   +5 more
europepmc   +1 more source

A matheuristic for the traveling salesman problem with positional consistency constraints

International Transactions in Operational Research, EarlyView.
Abstract We propose a matheuristic for the traveling salesman problem with positional consistency constraints, where we seek to generate a set of routes with minimum total cost, in which the nodes visited in more than one route (consistent nodes) must occupy the same relative position in all routes.
Luís Gouveia, Ana Paias, Mafalda Ponte
wiley   +1 more source

Deep learning assisted high‐resolution microscopy image processing for phase segmentation in functional composite materials

Journal of Microscopy, EarlyView.
Abstract In the domain of battery research, the processing of high‐resolution microscopy images is a challenging task, as it involves dealing with complex images and requires a prior understanding of the components involved. The utilisation of deep learning methodologies for image analysis has attracted considerable interest in recent years, with ...
Ganesh Raghavendran, Bing Han, Fortune Adekogbe, Shuang Bai, Bingyu Lu, William Wu, Minghao Zhang, Ying Shirley Meng   +7 more
wiley   +1 more source