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Metabolic Engineering, 2020
Escherichia coli is an ideal choice for constructing synthetic methylotrophs capable of utilizing the non-native substrate methanol as a carbon and energy source. All current E. coli-based synthetic methylotrophs require co-substrates. They display variable levels of methanol-carbon incorporation due to a lack of native regulatory control of ...
Maciek Antoniewicz +2 more
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Escherichia coli is an ideal choice for constructing synthetic methylotrophs capable of utilizing the non-native substrate methanol as a carbon and energy source. All current E. coli-based synthetic methylotrophs require co-substrates. They display variable levels of methanol-carbon incorporation due to a lack of native regulatory control of ...
Maciek Antoniewicz +2 more
exaly +3 more sources
Current Opinion in Biotechnology, 2015 Synthetic methylotrophy is the development of non-native methylotrophs that can utilize methane and methanol as sole carbon and energy sources or as co-substrates with carbohydrates to produce metabolites as biofuels and chemicals. The availability of methane (from natural gas) and its oxidation product, methanol, has been increasing, while prices have
Eleftherios Papoutsakis +2 more
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Synthetic methylotrophy in Escherichia coli and its industrial applications
2023Anthropogenic climate change, driven by greenhouse gas emissions, is one of humanity’s major challenges in the 21st century. Fossil-powered chemical pro-duction is a major contributor to global greenhouse gas emissions and is re-sponsible for about 1 billion metric tons of carbon dioxide equivalents annually.
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Synthetic methylotrophy: Engineering microbes for sustainable biomanufacturing from C1 compounds
Systems Microbiology and BiomanufacturingVaibhav Vinod Phokmare +2 more
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Foundational Tools for Synthetic Methylotrophy in Saccharomyces cerevisiae
2023The global expansion of biomanufacturing is currently limited by the availability of sugar-based microbial feedstocks. One-carbon feedstocks, like methanol, present an enticing alternative to sugar because they can be produced from organic waste, atmospheric carbon dioxide and hydrocarbons.
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Abstract Bacillus methanolicus represents a thermophilic methylotroph whose methanol utilization depends on plasmid-encoded genes. It serves as a unique model for deciphering plasmid-dependent methylotrophy and an ideal chassis for low-carbon biomanufacturing using CO2-derived C1 substrates.
Pan Liu +14 more
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Pan Liu +14 more
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