Results 81 to 90 of about 1,171,790 (266)
In Vitro Production and Screening of DNA Polymerase η Mutants for Catalytic Diversity
BioTechniques, 2002 Mutant DNA polymerases have become an increasingly important tool in biotechnology. The ability to examine the activity and specific properties of enzymes has a crucial role in the characterization of the enzyme.
Eitan Glick +2 more
doaj +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
FoodsOzonated vegetable oils are increasingly recognized as bioactive agents with both antimicrobial and functional properties, attracting growing interest for their potential applications in food systems.
Paula Dominguez-Lacueva +2 more
doaj +1 more source
STRUCTURAL APPROACH FOR NUMERICAL INTERNAL STRAIN MODELLING OF CONGLOMERATE STRUCTURES
Architecture and Engineering, 2017 The paper defines the principles for structure-simulation modelling of conglomerate material properties by an example of cement compositions. The constitutive essence of the principles consists in generation of hierarchical models of individual ...
Aleksey Kharitonov
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
A Formal Modeling Framework for Time-Aware Cyber–Physical Systems of Systems
SystemsCyber–Physical Systems of Systems (CPSoS) integrate autonomous constituent systems to accomplish complex missions. Nonetheless, decentralized coordination and continuous evolution create intricate dependencies that make behavior difficult to analyze ...
Riad Helal +3 more
doaj +1 more source
Systems-Level Properties of EGFR-RAS-ERK Signaling Amplify Local Signals to Generate Dynamic Gene Expression Heterogeneity. [PDF]
Cell Syst, 2020 Davies AE +11 more
europepmc +1 more source
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source