Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant [PDF]
BMC Genomics, 2019 Background Integration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research.
Huixin Gang +5 more
doaj +5 more sources
Characterization of fab2 T-DNA insertion mutants in terms of fatty acid composition and plant phenotype [PDF]
Plant Signaling & Behavior, 2023 Fatty acid biosynthesis 2 (FAB2) is an essential enzyme responsible for the synthesis of unsaturated fatty acids in chloroplast membrane lipids found in leaves and triacylglycerols (TAG) in seeds.
Mid-Eum Park +3 more
doaj +2 more sources
Characterization of T-Circles and Their Formation Reveal Similarities to Agrobacterium T-DNA Integration Patterns [PDF]
Frontiers in Plant Science, 2022 Agrobacterium transfers T-DNA to plants where it may integrate into the genome. Non-homologous end-joining (NHEJ) has been invoked as the mechanism of T-DNA integration, but the role of various NHEJ proteins remains controversial.
Kamy Singer +3 more
doaj +2 more sources
Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis [PDF]
BMC Genomics, 2021 Background Experimental proof of gene function assignments in plants is based on mutant analyses. T-DNA insertion lines provided an invaluable resource of mutants and enabled systematic reverse genetics-based investigation of the functions of Arabidopsis
Boas Pucker +2 more
doaj +2 more sources
Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing [PDF]
Frontiers in Plant Science, 2023 The commercialization of GE crops requires a rigorous safety assessment, which includes a precise DNA level characterization of inserted T-DNA. In the past, several strategies have been developed for identifying T-DNA insertion sites including, Southern ...
Eric Maina Magembe +5 more
doaj +2 more sources
T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data [PDF]
Genome BiologyAgrobacterium-mediated plant transformation, which enables the delivery of DNA using transfer DNA (T-DNA) binary vectors, is an essential technique in plant research.
Hongwoo Lee, Pil Joon Seo
doaj +2 more sources
The complex architecture and epigenomic impact of plant T-DNA insertions. [PDF]
PLoS Genetics, 2019 The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant ...
Florian Jupe +10 more
doaj +2 more sources
TDNAscan: A Software to Identify Complete and Truncated T-DNA Insertions [PDF]
Frontiers in Genetics, 2019 Transfer (T)-DNA insertions in mutants isolated from forward genetic screens are typically identified through thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), inverse PCR, or plasmid rescue.
Liang Sun +6 more
doaj +2 more sources
The rolC gene of agrobacteria: towards the understanding of its functions
Биотехнология и селекция растений, 2021 Agrobacterium rhizogenes Conn is a soil bacterium, which can transform plants by inserting a plasmid fragment into the plant genome. This fragment contains a “root locus”: four genes that cause root overgrowth of the transformed plant, the so-called ...
G. V. Khafizova, T. V. Matveeva
doaj +1 more source
High-Throughput Analysis of T-DNA Location and Structure Using Sequence Capture. [PDF]
PLoS ONE, 2015 Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput ...
Soichi Inagaki +3 more
doaj +1 more source