Results 11 to 20 of about 2,045,259 (260)
Microbiology Spectrum, 2022 Treponema pallidum can invade any organ, and T. pallidum DNA can be detected in various tissues and fluids. However, the knowledge of the presence and loads of T. pallidum DNA in urine is limited.
Cuini Wang +7 more
doaj +1 more source
T-DNA-genome junctions form early after infection and are influenced by the chromatin state of the host genome. [PDF]
PLoS Genetics, 2017 Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome.
Shay Shilo +5 more
doaj +1 more source
T-DNA Binary Vectors and Systems [PDF]
Plant Physiology, 2008 For more than two decades, scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants. Initial technologies to introduce genes of interest (goi) into Agrobacterium involved complex microbial genetic methodologies that inserted these goi into the transfer DNA (T-DNA) region of large tumor-inducing plasmids (Ti ...
Lan-Ying, Lee, Stanton B, Gelvin
openaire +2 more sources
Molecular Therapy: Nucleic Acids, 2018 The interaction of chemical and physical agents with genetic material can lead to almost 80 different DNA damage formations. The targeted intentional DNA damage by radiotherapy or chemotherapy is a front-line anticancer therapy. An interstrand cross-link
Boleslaw T. Karwowski
doaj +1 more source
Paramutation-like interaction of T-DNA loci in Arabidopsis. [PDF]
PLoS ONE, 2012 In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations.
Weiya Xue +7 more
doaj +1 more source
The Plant Journal, 1991 SummaryThe characteristics of Agrobacterium‐mediated plant transformation are such that the T‐DNA can be used as an insertional mutagen and, by extension, a gene tag. An increasing number of mutants have been obtained as a result of T‐DNA insertion and the versatility of this experimental system can be exploited further to tag sequences of DNA which ...
Walden, Richard +2 more
openaire +2 more sources
Deletions within intronic
The Plant Journal, 2023 SUMMARY Agrobacterium‐mediated transformation enables random transfer‐DNA (T‐DNA) insertion into plant genomes. T‐DNA insertion into a gene's exons, introns or untranscribed regions close to the start or stop codon can disrupt gene function.
Karen Thulasi Devendrakumar +3 more
openaire +2 more sources
Scientific Reports, 2018 The Schizosaccharomyces pombe sirtuin Hst4, functions in the maintenance of genome stability by regulating histone H3 lysine56 acetylation (H3K56ac) and promoting cell survival during replicative stress.
Lahiri Konada +3 more
doaj +1 more source
Computational and Structural Biotechnology Journal, 2023 Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai +2 more
doaj +1 more source
Screening of Transgenic Plants by Amplification of Unknown Genomic DNA Flanking T-DNA
BioTechniques, 1999 For the screening of transfer DNA (T-DNA) integration in transgenic plant material, we developed a method based on specific amplification of genomic plant DNA flanking T-DNA borders.
Diego Spertini +2 more
doaj +1 more source