Results 201 to 210 of about 2,045,259 (260)

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

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Identification and characterization of T-DNA inserts by T-DNA fingerprinting

Euphytica, 2002
A T-DNA fingerprinting method is presented based on amplified fragment length polymorphism with an anchored polymerase chain reaction step. This method allows discrimination between different T-DNA inserts in stably transformed plants. The technique was evaluated by analyzing 51 transgenic Arabidopsis lines that had been characterized in detail by ...
I. Theuns, P. Windels, S. De Buck, A. Depicker, E. Van Bockstaele, M. De Loose   +5 more
openaire   +1 more source

T-DNA insertion mutagenesis.

2012
This chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also
Sangwan, Rajbir S., Ochatt, Sergio, Nava-Saucedo, José-Edmundo, Sangwan-Norreel, Brigitte   +3 more
openaire   +2 more sources

T-DNA integration: Pol θ controls T-DNA integration

Nature Plants, 2016
The mechanism for T-DNA integration, a critical step of Agrobacterium-mediated transgenesis, remains poorly understood. Now, a study based on mutant analysis shows that Pol θ controls T-DNA integration and generates error-prone footprints at integration sites.
openaire   +2 more sources

T-DNA Mutagenesis in Arabidopsis

2003
Insertional mutagenesis is a basic genetic tool that allows for a rapid identification of the tagged genes responsible for a particular phenotype. Transposon and Agrobacterium-mediated DNA integration are the most commonly used biological mutagens in plants.
Jose M, Alonso, Anna N, Stepanova
openaire   +2 more sources

T-DNA mutagenesis in Brachypodium distachyon

Journal of Experimental Botany, 2011
During the past decade, Brachypodium distachyon has emerged as an attractive experimental system and genomics model for grass research. Numerous molecular tools and genomics resources have already been developed. Functional genomics resources, including mutant collections, expression/tiling microarray, mapping populations, and genome re-sequencing for ...
Vera, Thole, Antoine, Peraldi, Barbara, Worland, Paul, Nicholson, John H, Doonan, Philippe, Vain   +5 more
openaire   +2 more sources

T-DNA Transfer to Maize Plants

Molecular Biotechnology, 1999
Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on shoots of young maize seedlings ...
W H, Shen, J, Escudero, B, Hohn
openaire   +3 more sources

T-DNA insertional mutagenesis in Arabidopsis

Plant Molecular Biology, 1992
T-DNA insertion mutagenesis is one of those recently developed genetic techniques which can be expected to have a major impact in plant molecular biology [17, 50, 87]. Unlike conventional reviews, this brief outlook intends to give a practical insight into current problems and future applications of T-DNA tagging by underlining the potential of the ...
C, Koncz, K, Németh, G P, Rédei, J, Schell   +3 more
openaire   +2 more sources

Reassessing T-DNA insertions

Trends in Plant Science, 1998
Agrobacterium is a marvellous organism for plant genetic engineering, but we would do well to remember that it has evolved to serve its own ends and not ours. In last month's issue of Trends in Plant Science, Natasha Smith[1xMore T-DNA than meets the eye. Smith, N. Trends Plant Sci.
openaire   +1 more source

T‐DNA integration patterns in co‐transformed plant cells suggest that T‐DNA repeats originate from co‐integration of separate T‐DNAs

The Plant Journal, 1997
Nicotiana protoplasts and Arabidopsis leaf discs or roots were co‐cultivated with two Agrobacterium strains each carrying a different T‐DNA. Co‐transformed plants were selected and the integration of the different T‐DNAs was analysed at the genetic and genomic level.
M, De Neve, S, De Buck, A, Jacobs, M, Van Montagu, A, Depicker   +4 more
openaire   +2 more sources