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The Plant Journal, 1999
After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences ...
S, De Buck +3 more
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After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyzed by PCR, Arabidopsis root cells were co-transformed with two different T-DNAs with distinct sequences ...
S, De Buck +3 more
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2003
T-DNA activation tagging is a method to generate dominant mutations in plants or plant cells by random insertion of a T-DNA carrying constitutive enhancer elements, which can cause transcriptional activation of flanking plant genes. The method consists of generating a large number of transformed plants or plant cells using a specialized T-DNA construct,
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T-DNA activation tagging is a method to generate dominant mutations in plants or plant cells by random insertion of a T-DNA carrying constitutive enhancer elements, which can cause transcriptional activation of flanking plant genes. The method consists of generating a large number of transformed plants or plant cells using a specialized T-DNA construct,
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1991
The products of genes located on T-DNA, or Transferable DNA segments, of Ti and Ri plasmids from plant-pathogenic Agrobacteria are the immediate cause of the abnormal growth known as “Crown-gall’ s” and “Hairy-roots”. (For recent review see Weising et al., 1988.) “T-DNA” segments from Ti and Ri plasmids are transferred from the bacterial pathogen to ...
Csaba Koncz +3 more
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The products of genes located on T-DNA, or Transferable DNA segments, of Ti and Ri plasmids from plant-pathogenic Agrobacteria are the immediate cause of the abnormal growth known as “Crown-gall’ s” and “Hairy-roots”. (For recent review see Weising et al., 1988.) “T-DNA” segments from Ti and Ri plasmids are transferred from the bacterial pathogen to ...
Csaba Koncz +3 more
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New insights on T-DNA transfer
Trends in Microbiology, 1993polynucleotide vaccination. This suggests that the technology may be generally applicable. Thomas Braciale is correct in pointing out that the mechanism of antigen presentation after the injection of plasmid DNA has not been elucidated. However, based on our recent studies, it is not necessary to propose that antigen production by nonmuscle cells is ...
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Agrobacterium T-DNA integration: molecules and models
Trends in Genetics, 2004Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule (T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host genome. Whereas extensive work has revealed the biological mechanisms governing the production, Agrobacterium-to-plant cell transport and nuclear import of the Agrobacterium T-
Tzvi, Tzfira +3 more
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Termini and telomeres in T-DNA transformation
Plant Molecular Biology, 1994A T-DNA vector for plant transformation has been constructed in which the cloning site is located 9 bp from the right-border (RB) end and 27 bp from the left-border (LB) end. In this vector cloned DNA homologous to plant chromosomal sequences is located at the T-DNA termini, and will thus be exposed by even limited exonucleolysis in planta.
M, Chiurazzi, E R, Signer
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T‐DNA and Transposon Tagging in Aspen
Plant Biology, 2004Abstract: We have investigated the somatic activity of the maize Activator (Ac) element in haploid and diploid aspen with the objective of developing an efficient transposon‐based system for gene isolation in the model tree species Populus. It was shown that Ac is reinserted, frequently into or near coding regions in aspen, and therefore can be used ...
M, Fladung +3 more
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Transformation and T-DNA Mutagenesis
2015An efficient method to stably insert DNA into a genome is absolutely essential for a modern model system. In general, grasses are notoriously difficult to transform. Fortunately, Brachypodium distachyon has proven to be highly amenable to tissue culture and transformation. Indeed, current transformation methods make B. distachyon one of the easiest and
Mon Mandy Hsia, John P. Vogel
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1993
Upon cutting at the T-DNA borders the VirD2 protein of Agrobacterium tumefaciens is tightly (most probably covalently) attached to the 5’ end of the processed T-DNA. Therefore, it was proposed to be transferred into the plant cell together with the T-DNA, and to perform several functions within the plant cell: it may protect the T-DNA against nucleases,
Bruno Tinland +2 more
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Upon cutting at the T-DNA borders the VirD2 protein of Agrobacterium tumefaciens is tightly (most probably covalently) attached to the 5’ end of the processed T-DNA. Therefore, it was proposed to be transferred into the plant cell together with the T-DNA, and to perform several functions within the plant cell: it may protect the T-DNA against nucleases,
Bruno Tinland +2 more
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Mechanisms of T-DNA integration
2008T-DNA integration is the final step of the transformation process. During this step, the T-DNA, which traveled as a single-stranded DNA molecule from the bacterial cell through the host-cell cytoplasm into the nucleus, must covalently attach itself to the host to its point of integration in the host genome, to be stripped of some, if not all, of its ...
Alicja Ziemienowicz +2 more
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