Results 121 to 130 of about 458,059 (348)

In situ subcellular localization of epitope‐tagged human and rabbit carboxylesterases [PDF]

open access: bronze, 1998
Philip M. Potter   +4 more
openalex   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Apports et limites du marquage individuel dans la connaissance de l'écobiologie du chevaine (Leuciscus cephalus)

open access: yesKnowledge and Management of Aquatic Ecosystems, 1997
Depuis 1991, des marquages individuels ont été effectués sur des chevaines (Leuciscus cephalus) de la rivière Boutonne, affluent de la Charente, afin d'améliorer les connaissances écobiologiques de l'espèce qui restent très fragmentaires pour les ...
LE LOUARN H., BAGLINIÈRE J. L.
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Online Language Education: Principles of Creating a Marked Corpus of Learners’ Mistakes

open access: yesВиртуальная коммуникация и социальные сети
Today, academic corpora are a matter of great interest to many linguists. The article examines the importance of academic corpora in modern linguistics and pedagogy as a tool for identifying typical errors in the speech of foreign language students. They
Iuliia S. Vinogradova   +3 more
doaj   +1 more source

Freedom and Restraint: Tags, Vocabularies and Ontologies

open access: yes, 2006
The benefit of metadata is widely recognized. However, the nature of that information and the method of production remains a topic of some debate. This division is most noticeable between those who believe in ’free tagging’, and those who prefer the more
Lawrence, K. Faith, schraefel, m. c.
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Investigating bacterial populations in styrene-degrading biofilters by 16S rDNA tag pyrosequencing [PDF]

open access: hybrid, 2014
Kevin Portune   +3 more
openalex   +1 more source

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