Results 211 to 220 of about 171,839 (292)

A General Workflow for Tangential Flow Filtration Perfusion Scale‐Up

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Tangential flow filtration (TFF) perfusion using hollow‐fiber modules offers substantial advantages over other mammalian cell retention technologies. Recently, increased awareness of Starling flow has helped to enhance filtration performance to similar, or even higher levels compared to alternative technologies.
Patrick Romann, Ken Lee, Venkatesh Natarajan, Daniel Ley, Claes Nymand Nilsson, David Garcia‐Munzer, Dominik Schieman, Mahbod Hajighasemi, Alex Zaytsev, Jason Friedman, Loic Chappuis, Tobias Habicher, Colin Jaques, Alex Cohen, Matthew Gagnon, Edward Chan, Daniel J. Karst, Philip Giller, Antony Sibilia, Andrew L. Zydney, Thomas K. Villiger   +20 more
wiley   +1 more source

"Inching closer to native anatomy: A CT scan based morphometric analysis of tunnels in all-inside ACL reconstruction". [PDF]

J Clin Orthop Trauma
Jindal I, Thakur K, Singh CM, Sood C, Mahajan S, Agrawal M.   +5 more
europepmc   +1 more source

Purification of Disassembled Capsid Protein by Hydroxyapatite Chromatography Enhances Virus‐Like Particle Recovery

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Virus‐like particles (VLPs) are a promising modality with extensive applications in prophylactic and therapeutic vaccine design. Human Papillomavirus (HPV) vaccines are one such example where VLP vaccine applications have led to the successful reduction in HPV‐associated diseases, such as cervical cancer.
Nicholas Samuelson, Nathaniel Parker, Shiyi Wang, Sara Daley, Aaron Cote, Michael A. Winters   +5 more
wiley   +1 more source

The 3d Mixed BF Lagrangian 1-Form: A Variational Formulation of Hitchin's Integrable System. [PDF]

Commun Math Phys
Caudrelier V, Harland D, Singh AA, Vicedo B.   +3 more
europepmc   +1 more source

Isolating T‐Cell Potent Lentiviral Vector Subpopulations through Rational Design of Nanofiber Anion‐Exchange Adsorbents

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Generation of CAR‐T cell therapies relies predominantly on lentiviral vectors (LVs). As in vivo CAR‐T trials progress, demand for larger quantities of higher‐potency LV is expected. However, primary capture by anion‐exchange chromatography (AIEX) remains a major manufacturing bottleneck from use of adsorbents not tailored to LVs, leading to ...
George Pamenter, Gyorgy Ovari, Anurag Kulkarni, Thomas Williams‐Fegredo, Carol Knevelman, Kyriacos Mitrophanous, Lee Davies, Farzad Foroutan, Matthew Townsend, Stephen Goldrick, Daniel G. Bracewell   +10 more
wiley   +1 more source

Left Bundle Branch Area Pacing Reverts Electromechanical Window Negativity to a Similar Extent as Biventricular Pacing. [PDF]

Pacing Clin Electrophysiol
Klein F, Daliry M, Smits KC, Bressi E, Deissler PM, van Koll J, Rijks JHJ, van Stipdonk AMW, Ter Bekke RMA, Grieco D, Manfuro C, Spiridigliozzi G, Casalese A, Luermans JGLM, Prinzen FW, Meiburg R, Rademakers LM, Vernooy K, Nguyên UC.   +18 more
europepmc   +1 more source

Process Analytical Technology (PAT) for Continuous Purification of mRNA via Precipitation and Tangential Flow Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani, Maria del Carme Pons Royo, Rohan Kadambi, Andrew Hatas, Steve Burcat, Tyler Arnold, Hasan Al‐Mahayni, Richard D. Braatz, Allan S. Myerson   +8 more
wiley   +1 more source

Democritus: Homotopy-Localized Causal Discourse Extraction from Language. [PDF]

Entropy (Basel)
Mahadevan S.
europepmc   +1 more source

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

Preoperative dynamic anterior tibial translation is not predictive of graft rupture after anterior cruciate ligament reconstruction. [PDF]

J Exp Orthop
Mazy D, Cance N, Angelelli L, Pineda T, James Dan M, Dejour DH.   +5 more
europepmc   +1 more source