Results 141 to 150 of about 89,555 (262)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Learning Preferences and Characteristics of Generation Z Students in Pediatric Nursing, Midwifery, Physiotherapy, Occupational Therapy, Radiology Assistance, and Paramedicine: Protocol for a Scoping Review. [PDF]

JMIR Res Protoc
Volešová I, Javornická D, Vrlová L, Adeboye OR, Prušová E, Kašparová P, Kisvetrová H, Bagnasco A, Steen M, Steven A.   +9 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The Pharmacy Society of Wisconsin Workforce Summit: A statewide, multidisciplinary approach to create a pharmacy workforce strategic roadmap. [PDF]

Am J Health Syst Pharm
Bakken BK, Hartkopf K, Schaafsma K, Hager D.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Automated paraffin embedding of tissue samples significantly reduces overall workflow time in routine pathology practice. [PDF]

Pathologica
Gambella A, Zito L, Bottaro C, Paudice M, Tacchino G, Accorrà V, Piccione C, Terrile M, Bevere AS, Massa B, Bozzano S, Grillo F, Mastracci L.   +12 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Lessons learned from three approaches to digital pathology system validation at a large academic medical institution. [PDF]

J Pathol Inform
Krishnan T, Garrett J, Henriksen J, Zhen D, Gonzalez-Cuyar L, Hosny K.   +5 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Brain extraction for fixed tissue banking: a technical report. [PDF]

Free Neuropathol
Wolf M, Beck A, Paredes L, Darcy S, Parra A, Taylor GA, Garrood M, Thorn EL, Sanctis C, Crary JF, Farrell K, McKenzie AT.   +11 more
europepmc   +1 more source