SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Dominion East Ohio and Natural Gas Workers Union, Service Employees\u27 International Union (SEIU), AFL-CIO, Local 555 (2001) [PDF]
core +1 more source
Morphological comparison between artificial intelligence-driven and manual CAD design in single tooth restoration: a preliminary study. [PDF]
Xie BY +8 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
New York City Health & Hospital Corporation and District Council 37, American Federation of State, County & Municipal Employees (AFSCME), AFL-CIO, Local 237, International Brotherhood of Teamsters (IBT) and 44 Division of Local 1199 Health & Human Services Employees Union, Service Employees International Union (SEIU), AFL-CIO (2000) [PDF]
core +1 more source
Keeping the Cares Together : How Animal Technicians Deal with Ethical Dilemmas in the Laboratory
Hanne Castberg Tresselt
openalex +1 more source
The Message or the Messenger: The Impact of Explanation Satisfaction and Source Characteristics on Perceptions of Experts and Verdicts. [PDF]
Gittings KL, Giffin CE, Salerno JM.
europepmc +1 more source
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
Regional Transit Service and Amalgamated Transit Union (ATU), Local 282 (1998) [PDF]
core +1 more source
Evaluation of beliefs, practices and importance among Health Sciences University students regarding hand hygiene. [PDF]
Terzioglu B +4 more
europepmc +1 more source

