Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The Effect of Situational Leadership on Healthcare Service Quality Through Crisis Management and Resources Availability: The Moderating Role of Leader Experience. [PDF]
Ghallab A, Sallam F.
europepmc +1 more source
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
A collaborative multi-university virtual model for global health leadership education integrating educational technology: a mixed methods evaluation. [PDF]
Kumar S, Zhou C, Withers M.
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Unmasking narcissistic leadership: a multilevel time-lagged study of the mediating role of employee resilience and the moderating role of supportive team climate. [PDF]
Li X, Hahn J.
europepmc +1 more source
Technology, responsiveness, and leadership [PDF]
openaire +1 more source
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
How hospital accreditation requirements bridge enablers for AI readiness: interpretative analysis of intersections in framework standards. [PDF]
Bellei EA +3 more
europepmc +1 more source

